Kurian G A, Paddikkala J
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur - 613 402, India.
Indian J Pharm Sci. 2010 Nov;72(6):745-52. doi: 10.4103/0250-474X.84585.
The present study investigate the protective effect of aqueous root extract of Desmodium gangeticum in preserving mitochondrial and sarcoplasmic ATPase during ischemia reperfusion injury. The isolated rat hearts in both drug and control group were subjected to warm ischemia (37°), followed by reperfusion with the Langendorff perfusion system. The aqueous root extract of Desmodium gangeticum (L) at a dose of 50 mg/kg body weight was found to be effective in the rat heart for the management of ischemic reperfusion injury. Physiological parameters were significantly (P<0.05) improved in drug treated rat hearts. Creatine phosphokinase in coronary perfusate found to be declined. Moreover, sarcoplasmic ATPase and mitochondrial enzymes were significantly (P<0.05) improved in drug treated rat hearts. In fact, histological analysis of the myocardium also suggested an improved ultra structure in Desmodium gangeticum treated rat heart. These results suggest that Desmodium gangeticum aqueous root extract can preserve the mitochondrial and sarcoplasmic ATPase in the myocardium, resulting in the improvement of cardiac function after ischemia reperfusion injury.
本研究探讨了恒河猴耳草水提物在缺血再灌注损伤期间对心肌线粒体和肌浆网ATP酶的保护作用。药物组和对照组的离体大鼠心脏均经历了37℃的热缺血，随后用Langendorff灌注系统进行再灌注。发现恒河猴耳草（L）水提物以50mg/kg体重的剂量对大鼠心脏管理缺血再灌注损伤有效。药物处理的大鼠心脏的生理参数有显著（P<0.05）改善。发现冠状动脉灌流液中的肌酸磷酸激酶下降。此外，药物处理的大鼠心脏中肌浆网ATP酶和线粒体酶有显著（P<0.05）改善。事实上，心肌的组织学分析也表明恒河猴耳草处理的大鼠心脏超微结构有所改善。这些结果表明，恒河猴耳草水提物可以保护心肌中的线粒体和肌浆网ATP酶，从而改善缺血再灌注损伤后的心脏功能。
