岗梅根乙酸乙酯提取物对大鼠心肌缺血再灌注损伤的抗氧化作用。
Antioxidant effects of ethyl acetate extract of Desmodium gangeticum root on myocardial ischemia reperfusion injury in rat hearts.
机构信息
School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu, India.
出版信息
Chin Med. 2010 Jan 22;5:3. doi: 10.1186/1749-8546-5-3.
BACKGROUND
This study aims to evaluate the antioxidant potential of the ethyl acetate extract of Desmodium gangeticum root for cardioprotection from ischemia reperfusion-induced oxidative stress.
METHODS
The in vitro antioxidant potential of the extract was in terms of hydroxyl radical scavenging activity, lipid peroxide scavenging activity, nitric oxide scavenging activity and diphenylpicrylhydrazyl radical scavenging activity. The in vivo antioxidant potential of the extract was assessed in an isolated rat heart model.
RESULTS
Free radicals were scavenged by the extract in a concentration-dependent manner within the range of the given concentrations in all models. Administration of the ethyl acetate extract of Desmodium gangeticum root (100 mg per kg body weight) before global ischemia caused a significant improvement of cardiac function and a decrease in the release of lactate dehydrogenase in coronary effluent, as well as the level of malondialdehyde in myocardial tissues.
CONCLUSION
The ethyl acetate extract of Desmodium gangeticum root protects the myocardium against ischemia-reperfusion-induced damage in rats. The effects of the extract may be related to the inhibition of lipid peroxidation.
背景
本研究旨在评估山蚂蝗根的乙酸乙酯提取物的抗氧化潜力，以保护心脏免受缺血再灌注引起的氧化应激损伤。
方法
提取物的体外抗氧化潜力是通过羟基自由基清除活性、脂质过氧化物清除活性、一氧化氮清除活性和二苯代苦味肼基自由基清除活性来评估的。提取物的体内抗氧化潜力在离体大鼠心脏模型中进行评估。
结果
自由基在所有模型中在所给浓度范围内以浓度依赖的方式被提取物清除。在全心缺血前给予山蚂蝗根的乙酸乙酯提取物（100mg/kg 体重）可显著改善心脏功能，并降低冠状流出液中乳酸脱氢酶的释放以及心肌组织中丙二醛的水平。
结论
山蚂蝗根的乙酸乙酯提取物可保护大鼠心肌免受缺血再灌注损伤。提取物的作用可能与抑制脂质过氧化有关。
Zotero 插件