Fang Wen, Xiao Liang-Liang, Bao Huai-En, Mu Rong
Department of Clinical Laboratory Sciences, Guiyang Medical College, Guiyang 550004, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Jun;29(3):188-90.
Two 20-day-old three-way crossed hybrid pigs were infected with 80000 Taenia solium or T. asiatica eggs, respectively. Immature cysticerci of the two species in liver were collected at 40 days after infection. The total proteins were separated by two-dimensional electrophoresis, and differentially expressed proteins were analyzed by Image-Master 2D Platinum 6.0 software. The results showed that there were (236 +/- 12) and (231 +/- 14) protein spots in 2D electrophoresis gel images of T. solium and T. asiatica, respectively, with 3 proteins up-regulated and 7 proteins down-regulated in T. solium cysticercus by 2-fold or more compared with those in T. asiatica cysticercus.
分别给两头20日龄的三元杂交猪感染80000个猪带绦虫或亚洲带绦虫虫卵。感染后40天收集两物种在肝脏中的未成熟囊尾蚴。通过二维电泳分离总蛋白，并用Image-Master 2D Platinum 6.0软件分析差异表达蛋白。结果显示，猪带绦虫和亚洲带绦虫的二维电泳凝胶图像中分别有(236±12)和(231±14)个蛋白点，与亚洲带绦虫囊尾蚴相比，猪带绦虫囊尾蚴中有3种蛋白上调、7种蛋白下调2倍或更多。
