基于循环酶扩增方法的石墨烯氧化物保护 DNA 探针在复杂生物样品中用于多重 microRNA 分析。
Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method.
机构信息
The Key Laboratory of Analytical Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
出版信息
Chem Commun (Camb). 2012 Jan 7;48(2):194-6. doi: 10.1039/c1cc15412e. Epub 2011 Oct 5.
PMID:21971052
Abstract
Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of graphene oxide for multiple fluorophores, this method can distinguish highly similar miRNA sequences and detect them simultaneously.
摘要
基于氧化石墨烯保护的 DNA 探针，我们开发了一种用于复杂生物样本中灵敏 miRNA 检测的循环酶扩增方法。通过利用氧化石墨烯对多种荧光团的淬灭特性，该方法能够区分高度相似的 miRNA 序列并同时进行检测。
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