Moore P A, Bettany A J, Brown J P
Department of Genetics, University of Glasgow, U.K.
Gene. 1990 Apr 30;89(1):85-92. doi: 10.1016/0378-1119(90)90209-a.
The Saccharomyces cerevisiae pyruvate kinase-encoding gene (PYK1) has been transformed back into yeast using a derivative of the multicopy vector, pJDB207. High levels of PYK1 expression in these transformants are limited by at least two separate mechanisms. Pyruvate kinase assays and polysome analyses demonstrate that the translation of the PYK1 mRNA is inhibited as its abundance increases. The abundance of the PYK1 mRNA per gene copy also decreases as the copy number of the PYK1 gene increases. This is the first report which demonstrates that a eukaryotic glycolytic gene is subject to dosage limitation at the translational level.
已使用多拷贝载体pJDB207的衍生物将酿酒酵母丙酮酸激酶编码基因（PYK1）转化回酵母中。这些转化体中PYK1的高水平表达受到至少两种独立机制的限制。丙酮酸激酶测定和多核糖体分析表明，随着PYK1 mRNA丰度的增加，其翻译受到抑制。随着PYK1基因拷贝数的增加，每个基因拷贝的PYK1 mRNA丰度也会降低。这是第一份证明真核糖酵解基因在翻译水平上受到剂量限制的报告。
