Mason A B, Buckley H R, Gorman J A
Department of Microbial Molecular Biology, Bristol-Myers, Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
J Bacteriol. 1993 May;175(9):2632-9. doi: 10.1128/jb.175.9.2632-2639.1993.
A DNA clone containing the putative Candida albicans enolase gene (ENO1) was isolated from a genomic DNA library. The sequenced insert contained a continuous open reading frame of 1,320 bp. The predicted 440-amino-acid protein is 78 and 76% identical, respectively, to Saccharomyces cerevisiae enolase proteins 1 and 2. Only one enolase gene could be detected in C. albicans genomic DNA by Southern analysis with a homologous probe. Northern (RNA) analysis detected a single, abundant C. albicans ENO1 transcript of approximately 1,600 nucleotides. When cells were grown on glucose, levels of ENO1 mRNA were markedly increased by comparison with ENO1 mRNA levels in cells grown on ethanol, a gluconeogenic carbon source. In contrast to this glucose-mediated transcriptional induction, the carbon source had no dramatic effect on the levels of enolase protein or enzyme activity in the C. albicans strains tested. These results suggest that posttranscriptional mechanisms are responsible for modulating expression of the C. albicans enolase gene.
从基因组DNA文库中分离出一个含有白色念珠菌烯醇化酶基因（ENO1）推测序列的DNA克隆。测序后的插入片段包含一个1320 bp的连续开放阅读框。预测的440个氨基酸的蛋白质与酿酒酵母烯醇化酶蛋白1和2分别有78%和76%的同源性。用同源探针进行Southern分析时，在白色念珠菌基因组DNA中只能检测到一个烯醇化酶基因。Northern（RNA）分析检测到一条约1600个核苷酸的单一且丰富的白色念珠菌ENO1转录本。当细胞在葡萄糖上生长时，与在乙醇（一种糖异生碳源）上生长的细胞相比，ENO1 mRNA水平显著增加。与这种葡萄糖介导的转录诱导相反，碳源对所测试的白色念珠菌菌株中烯醇化酶蛋白水平或酶活性没有显著影响。这些结果表明，转录后机制负责调节白色念珠菌烯醇化酶基因的表达。
