School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Theor Appl Genet. 2012 Feb;124(2):249-59. doi: 10.1007/s00122-011-1701-x. Epub 2011 Oct 5.
The high-density consensus map was constructed based on the GY14 × PI 183967 map from an inter-subspecific cross and the extended S94 × S06 map from an intra-subspecific cross. The consensus map was composed of 1,369 loci, including 1,152 SSR loci, 192 SRAP loci, 21 SCAR loci and one STS locus as well as three gene loci of fruit external quality traits in seven chromosomes, and spanned 700.5 cM, of which 682.7 cM (97.5%) were covered by SSR markers. The average genetic distance and physical interval between loci were 0.51 cM and ~268 kbp, respectively. Additionally, the physical position of the sequence-associated markers aligned along the assembled cucumber genome sequence established a relationship between genetic maps and cucumber genome sequence and to a great extent validated the order of markers in individual maps and consensus map. This consensus map with a high marker density and well-ordered markers is a saturated and reliable linkage map for genetic analysis of cucumber or the Cucurbitaceae family of plants.
高密度共识图谱是基于种间杂交的 GY14×PI 183967 图谱和种内杂交的 S94×S06 图谱构建的。共识图谱由 1369 个标记组成，包括 1152 个 SSR 标记、192 个 SRAP 标记、21 个 SCAR 标记和一个 STS 标记，以及七个染色体上三个果实外观品质性状的基因标记，共覆盖 700.5cM，其中 682.7cM（97.5%）由 SSR 标记覆盖。标记之间的平均遗传距离和物理间隔分别为 0.51cM 和~268kbp。此外，序列相关标记在组装的黄瓜基因组序列上的物理位置建立了遗传图谱与黄瓜基因组序列之间的关系，并在很大程度上验证了各个图谱和共识图谱中标记的顺序。该共识图谱具有高密度的标记和有序排列的标记，是黄瓜或葫芦科植物遗传分析的饱和且可靠的连锁图谱。
