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一个 HD-ZIP I 转录因子的 SNP 导致黄瓜（Cucumis sativus L.）的毛状体形态扭曲。

A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.).

机构信息

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

State Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300384, China.

出版信息

BMC Plant Biol. 2021 Apr 16;21(1):182. doi: 10.1186/s12870-021-02955-1.

DOI:10.1186/s12870-021-02955-1
PMID:33863289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8052656/
Abstract

BACKGROUND

Trichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line 'WD1', we identified an EMS-induced trichome abnormally developing mutant, nps, which exhibited smaller, denser and no pyramid-shaped head trichomes.

RESULTS

Using F and BC populations constructed from a cross between nps and '9930', the genetic analysis showed that the nps trait is controlled by a single recessive nuclear gene. We identified CsNps by map-based cloning with 576 individuals of the F population generated from the cross of nps and inbred line '9930'. The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 and nps was found in the second exon of Csa3G748220, a plant-specific class I HD-Zip gene. The result of allelism test also indicated that nps is a novel allelic mutant of Mict (Micro-trichome). Thus, nps was renamed mict-L130F. By comparing the transcriptome of mict-L130F vs WD1 and 06-2 (mict) vs 06-1 (wildtype, near-isogenic line of 06-2), several potential target genes that may be related to trichome development were identified.

CONCLUSIONS

Our results demonstrate that Mict-L130F is involved in the morphogenesis of trichomes. Map-based cloning of the Mict-L130F gene could promote the study of trichome development in cucumber.

摘要

背景

毛状体是分析细胞分化的极佳模式系统，在植物保护中起着重要作用。我们从黄瓜自交系 'WD1' 中鉴定出一个 EMS 诱导的毛状体异常发育突变体 nps，其表现为毛状体变小、变密且无金字塔形头部。

结果

利用 nps 与 '9930' 杂交构建的 F 和 BC 群体进行遗传分析表明，nps 性状受单个隐性核基因控制。我们通过基于图谱的克隆方法，利用 nps 与自交系 '9930' 杂交产生的 576 个 F1 群体个体，鉴定出 CsNps。CsNps 位于 3 号染色体上的一个 13.4-kb 基因组区域，该区域包含三个预测基因。序列分析表明，在自交系 '9930' 和 nps 之间的第二外显子中，仅发现一个单核苷酸突变（C→T），这是一个植物特异性的 I 类 HD-Zip 基因。等位基因测试的结果也表明，nps 是 Mict（微毛状体）的一个新等位基因突变体。因此，nps 被重新命名为 mict-L130F。通过比较 mict-L130F 与 WD1 和 06-2（mict）与 06-1（野生型，06-2 的近等基因系）的转录组，鉴定出几个可能与毛状体发育相关的潜在靶基因。

结论

我们的结果表明，Mict-L130F 参与毛状体的形态发生。Mict-L130F 基因的图谱克隆可以促进黄瓜毛状体发育的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/23521da1d560/12870_2021_2955_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/af1a7a39a21d/12870_2021_2955_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/f81f56f2c76d/12870_2021_2955_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/adca535ace4a/12870_2021_2955_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/85c49468e22c/12870_2021_2955_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/73f347c65392/12870_2021_2955_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/23521da1d560/12870_2021_2955_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/af1a7a39a21d/12870_2021_2955_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/f81f56f2c76d/12870_2021_2955_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/adca535ace4a/12870_2021_2955_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/85c49468e22c/12870_2021_2955_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/73f347c65392/12870_2021_2955_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/48fc/8052656/23521da1d560/12870_2021_2955_Fig6_HTML.jpg

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