Faculty of Sciences, Charles University in Prague, 128 43 Prague 2, Czech Republic.
J Biomed Mater Res A. 2011 Dec 15;99(4):676-83. doi: 10.1002/jbm.a.33218. Epub 2011 Oct 4.
Electrospun polymeric nanofiber materials doped with 5,10,15,20-tetraphenylporphyrin (TPP) photosensitizer were prepared from four different polymers and were characterized with microscopic methods, steady-state, and time-resolved fluorescence and absorption spectroscopy. The polymers used included polyurethane Larithane™ (PUR), polystyrene (PS), polycaprolactone (PCL), and polyamide 6 (PA6). The antibacterial activity of all nanofiber materials against E. coli was activated by visible light and it was dependent on oxygen permeability/diffusion coefficients and the diameter of the polymeric nanofibers. This activity is based on oxidation ability of singlet oxygen O₂(¹Δ(g)) that is generated upon irradiation. All tested nanofiber materials exhibited prolonged antibacterial properties, even in the dark after long-duration irradiation. The post-irradiation effect was explained by the photogeneration of H₂O₂, which provided the material with long-lasting antibacterial properties.
掺杂 5,10,15,20-四苯基卟啉（TPP）敏化剂的电纺聚合物纳米纤维材料由四种不同的聚合物制备而成，并通过微观方法、稳态和时间分辨荧光和吸收光谱进行了表征。所用的聚合物包括聚氨基甲酸酯 Larithane™（PUR）、聚苯乙烯（PS）、聚己内酯（PCL）和聚酰胺 6（PA6）。所有纳米纤维材料对大肠杆菌的抗菌活性都可以通过可见光激活，并且取决于氧气渗透率/扩散系数和聚合物纳米纤维的直径。这种活性基于照射时产生的单线态氧 O₂(¹Δ(g))的氧化能力。所有测试的纳米纤维材料都表现出持久的抗菌性能，即使在长时间照射后的黑暗中也是如此。后照射效应可以通过 H₂O₂的光生成来解释，这为材料提供了持久的抗菌性能。
