Yang Gui-li, Yang Jie, Zhang Jun, Xu Yu-ying, Wei Qiao-hui, Sun Xiang-yu, Gu Xiao-min, Zhang Yin, Ding Qiu-ping, Zheng Yi-fan, Yang Jun, Zhu Xin-qiang
Department of Toxicology, Zhejiang University School of Public Health, Hangzhou 310058, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 May;29(5):321-3.
To observe the effects of multiwall carbon nano-onions (MWCNOs) on platelet aggregation and hemostatic function.
The platelet aggregation was determined with Born's method at different concentration of MWCNOs (0, 0.2, 2.0, 20.0 microg/ml) in vitro. Twenty male SD rats were randomly divided into 4 groups which were exposed to 0, 2, 4 and 8 mg/kg MWCNOs, respectively. Then platelet count, platelet aggregation, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), bleeding time (BT) and platelet count (PC) were measured at 12 h after receiving tail intravenous injection of MWCNOs. The effects of MWCNOs (4 mg/kg) on platelet aggregation and platelet count at different time points were observed.
In vitro, MWCNOs exhibited the potent inhibitory effects on rat platelet aggregation caused by ADP in a concentration-dependent manner. The platelet aggregation in the highest dosage of 20.0 microg/ml group was 50.0% +/- 6.9% which was significantly lower than that (73.2% +/- 4.3%) in control group (P<0.01). In vivo, the highest inhibitory was up to 20.4%, but there was no significant difference, as compared with control group. MWCNOs did not affect the APTT, PT, TT, BT and PC.
Under this experimental condition, MWCNOs might inhibit platelet aggregation but not affect hemostatic function.
观察多壁碳纳米洋葱（MWCNOs）对血小板聚集和止血功能的影响。
采用Born法在体外不同浓度（0、0.2、2.0、20.0微克/毫升）的MWCNOs条件下测定血小板聚集情况。将20只雄性SD大鼠随机分为4组，分别给予0、2、4和8毫克/千克的MWCNOs。在尾静脉注射MWCNOs 12小时后，检测血小板计数、血小板聚集、活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、凝血酶时间（TT）、出血时间（BT）和血小板计数（PC）。观察MWCNOs（4毫克/千克）在不同时间点对血小板聚集和血小板计数的影响。
在体外，MWCNOs对ADP诱导的大鼠血小板聚集具有显著的抑制作用，呈浓度依赖性。最高剂量20.0微克/毫升组的血小板聚集率为50.0%±6.9%，显著低于对照组（73.2%±4.3%）（P<0.01）。在体内，最高抑制率达20.4%，但与对照组相比无显著差异。MWCNOs对APTT、PT、TT、BT和PC无影响。
在此实验条件下，MWCNOs可能抑制血小板聚集，但不影响止血功能。
