Li Peng, Fang Jian-Qiao, Zhou Ya-Feng
Department of Massage, The Second Affiliated Hospital of Zhejiang University of TCM, Hangzhou 310005, China.
Zhongguo Zhen Jiu. 2011 Sep;31(9):821-6.
To observe the therapeutic effect of acupoint application with Leima type II plaster on collagen-induced arthritis (CIA) in rats and probe its mechanism.
Bovine type II collagen was injected intradermally into the middle line of the back to induce CIA model with 48 Wistar rats. Then the rats were randomly divided into a model control group (group A), a matrix control group (group B), acupoint application group with plaster of low concentration (group C) and high concentration plaster group (group D), 12 rats in each group. Group C and group D were treated with low and high concentration of Leima type II plaster, and "Shenzhu" (GV 12), "Zhiyang" (GV 9) and "Mingmen" (GV 4) were selected, each application for about 15 hours, once each day for 30 days. Group B was used the same method of acupoint application except using non-drug matrix plaster, and group A was not given any treatment. The morphous and the histopathological changes of affection joint were observed.
The paw edema volume after 30 days treatment in group C was significantly lower than that in group B (P < 0.01), and the anti-type II collagen antibody level after 15 days treatment in group C was significantly lower than that in group A (P < 0.05), and the synoviocytes proliferation of the knee joint in group C was significantly lower than that in group A and group B (both P < 0.01). The paw edema volume after 25 days treatment, arthritic index after 20 days treatment, pathological change of the paw and the synoviocytes proliferation of the knee joint in group D were significantly lower than those in group A and group B (P < 0.01, P < 0.05), and the anti-type II collagen antibody level after 15 days treatment in group D was significantly lower than that in group A (P < 0.05), and the paw edema volume and the arthritic index after 25 days treatment in group D were significantly lower than those in group C (P < 0.05, P < 0.01).
Acupoint application with Leima type II plaster has a good therapeutic effect on CIA rats and the protective mechanism is related to the reduction of anti-type II collagen antibody level so as to carry out anti-inflammatory effect and immunosuppression.
观察雷马Ⅱ型膏药穴位贴敷对大鼠胶原诱导性关节炎（CIA）的治疗作用并探讨其机制。
将牛Ⅱ型胶原皮内注射到48只Wistar大鼠背部中线诱导CIA模型。然后将大鼠随机分为模型对照组（A组）、基质对照组（B组）、低浓度膏药穴位贴敷组（C组）和高浓度膏药组（D组），每组12只。C组和D组分别用低、高浓度雷马Ⅱ型膏药贴敷，选取“神阙”（GV 12）、“至阳”（GV 9）和“命门”（GV 4），每次贴敷约15小时，每天1次，共30天。B组除使用不含药物的基质膏药外，采用相同的穴位贴敷方法，A组不给予任何治疗。观察病变关节的形态及组织病理学变化。
C组治疗30天后足爪肿胀体积显著低于B组（P<0.01），C组治疗15天后抗Ⅱ型胶原抗体水平显著低于A组（P<0.05），C组膝关节滑膜细胞增殖显著低于A组和B组（均P<0.01）。D组治疗25天后足爪肿胀体积、治疗20天后关节炎指数、足爪病理变化及膝关节滑膜细胞增殖均显著低于A组和B组（P<0.01，P<0.05），D组治疗15天后抗Ⅱ型胶原抗体水平显著低于A组（P<0.05），D组治疗25天后足爪肿胀体积和关节炎指数显著低于C组（P<0.05，P<0.01）。
雷马Ⅱ型膏药穴位贴敷对CIA大鼠有良好的治疗作用，其保护机制与降低抗Ⅱ型胶原抗体水平从而发挥抗炎和免疫抑制作用有关。
