Kelling C L, Staudinger W L, Rhodes M B
Am J Vet Res. 1978 Dec;39(12):1955-7.
An indirect solid-phase microradioimmunoassay (IRIA) was developed for detection and quantitation of antibodies to pseudorabies virus (PRV) in swine serum. Qualitative results of the IRIA compared closely with results of the serum neutralization test (NT) and the microimmunodiffusion test (MIDT). The IRIA was more sensitive than the NT for detection of antibodies to PRV in swine serum. The IRIA result is expressed numerically. With the IRIA and NT, antibody to PRV was first detectable in 3 experimentally infected pigs at 9 days after inoculation. With MIDT, antibody was detected in the 3 experimentally infected pigs at 9 days after inoculation. With the MIDT, antibody was detected in the 3 experimentally infected pigs at 7, 8, and 9 days after inoculation. The IRIA results are obtainable within a few hours; the NT and MIDT require 48 hours for completion.
建立了一种间接固相微放射免疫分析法（IRIA），用于检测和定量猪血清中伪狂犬病病毒（PRV）抗体。IRIA的定性结果与血清中和试验（NT）和微量免疫扩散试验（MIDT）的结果密切相关。在检测猪血清中PRV抗体方面，IRIA比NT更敏感。IRIA结果以数字形式表示。使用IRIA和NT，在3头实验感染猪接种后第9天首次检测到PRV抗体。使用MIDT，在3头实验感染猪接种后第9天检测到抗体。使用MIDT，在3头实验感染猪接种后第7、8和9天检测到抗体。IRIA结果可在数小时内获得；NT和MIDT需要48小时才能完成。
