用于检测猪伪狂犬病病毒抗体的间接酶免疫测定法的建立与评估
Development and evaluation of an indirect enzyme immunoassay for detection of porcine antibodies to pseudorabies virus.
作者信息
Afshar A, Wright P F, Dulac G C
出版信息
Can J Vet Res. 1986 Jul;50(3):422-6.
An indirect enzyme immunoassay is described for detection of porcine serum antibody to pseudorabies virus. The analytical sensitivity of the enzyme immunoassay was found to be approximately 4.5 log 4 X 10 (5120 times) greater than the serum neutralization test, based on parallel end point titrations. The diagnostic sensitivity of the enzyme immunoassay was comparable or superior to that of the serum neutralization, based on the earliest detectable antibody after infection of swine with pseudorabies virus by intranasal or intrauterine routes or by contact with infected pigs. The enzyme immunoassay, at a screening dilution of 1:20, gave 100% agreement with ELISA results provided with a U.S. Department of Agriculture-Animal and Plant Health Inspection Service proficiency panel of 40 sera. One serum having demonstrable antibody by the enzyme immunoassay was seronegative by the serum neutralization test.
本文描述了一种用于检测猪血清中伪狂犬病病毒抗体的间接酶免疫测定法。基于平行终点滴定法，发现该酶免疫测定法的分析灵敏度比血清中和试验约高4.5 log 4×10（5120倍）。基于猪经鼻内、子宫内途径感染伪狂犬病病毒或与感染猪接触后最早可检测到的抗体，该酶免疫测定法的诊断灵敏度与血清中和试验相当或更高。在1:20的筛查稀释度下，该酶免疫测定法与美国农业部动植物卫生检验局提供的40份血清的酶联免疫吸附测定（ELISA）结果完全一致。有一种血清通过酶免疫测定法显示有可检测抗体，但通过血清中和试验呈血清阴性。
Zotero 插件