González-Mosquera A, Seoane J, García-Caballero L, López-Jornet P, García-Caballero T, Varela-Centelles P
Stomatology Department, School of Medicine and Dentistry, University of Santiago de Compostela, Entrerríos s/n, 15782 Santiago de Compostela, Spain.
Br J Oral Maxillofac Surg. 2012 Sep;50(6):508-12. doi: 10.1016/j.bjoms.2011.09.006. Epub 2011 Oct 4.
Our aim was to assess wounds made by lasers (CO(2) and Er,Cr:YSGG) for their epithelial architectural changes and width of damage. We allocated 60 Sprague-Dawley(®) rats into groups: glossectomy by CO(2) laser at 3 different wattages (n=10 in each); glossectomy by Er,Cr:YSGG laser at two different emissions (n=10 in each), and a control group (n=10). Histological examination assessed both prevalence and site of thermal artefacts for each group. Both lasers (CO(2) and Er,Cr:YSGG) caused the same type of cytological artefacts. The 3W Er,Cr:YSGG laser produced the fewest cytological artefacts/specimen, and was significantly different from the other experimental groups: 3W CO(2) laser (95% CI=0.8 to 1.0); the 6W CO(2) laser (95% CI=0.1 to 2.0) and the 10W CO(2) laser (95% CI=1.1 to 3.0). CO(2) lasers (3-10W) generate epithelial damage that can simulate dysplastic changes with cytological atypia that affects mainly the basal and suprabasal layers. Irradiation with Er,CR:YSGG laser (2-4W) produces significantly fewer cellular artefacts and less epithelial damage, which may be potentially useful for biopsy of oral mucosa.
我们的目的是评估激光（二氧化碳激光和铒铬钇钪石榴石激光）造成的伤口的上皮结构变化和损伤宽度。我们将60只斯普拉格-道利（®）大鼠分为几组：分别用三种不同功率的二氧化碳激光进行舌切除术（每组10只）；分别用两种不同发射功率的铒铬钇钪石榴石激光进行舌切除术（每组10只），以及一个对照组（10只）。组织学检查评估了每组热伪像的发生率和部位。两种激光（二氧化碳激光和铒铬钇钪石榴石激光）造成的细胞学伪像类型相同。3瓦铒铬钇钪石榴石激光产生的细胞学伪像/标本最少，与其他实验组有显著差异：3瓦二氧化碳激光（95%可信区间=0.8至1.0）；6瓦二氧化碳激光（95%可信区间=0.1至2.0）和10瓦二氧化碳激光（95%可信区间=1.1至3.0）。3 - 10瓦的二氧化碳激光会造成上皮损伤，可模拟发育异常变化并伴有细胞学异型性，主要影响基底层和基底上层。用2 - 4瓦铒铬钇钪石榴石激光照射产生的细胞伪像明显较少，上皮损伤也较小，这可能对口腔黏膜活检有潜在用途。
