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1
Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study.基层应用 Xpert MTB/RIF 检测诊断结核分枝杆菌感染和耐药的可行性、诊断准确性和效果：一项多中心实施研究。
Lancet. 2011 Apr 30;377(9776):1495-505. doi: 10.1016/S0140-6736(11)60438-8. Epub 2011 Apr 18.
2
Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.资源匮乏国家的结核病实验室诊断：挑战与机遇。
Clin Microbiol Rev. 2011 Apr;24(2):314-50. doi: 10.1128/CMR.00059-10.
3
Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.比较 Xpert MTB/RIF 检测与 IS6110-TaqMan 实时 PCR 检测在呼吸道和非呼吸道标本中直接检测结核分枝杆菌的效果。
J Clin Microbiol. 2011 May;49(5):1772-6. doi: 10.1128/JCM.02157-10. Epub 2011 Mar 16.
4
Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples.快速、高效检测呼吸道和非呼吸道样本中的结核分枝杆菌。
Int J Tuberc Lung Dis. 2011 Apr;15(4):553-5. doi: 10.5588/ijtld.10.0497.
5
Evaluation of the Cepheid Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.评价 Cepheid Xpert MTB/RIF assay 直接检测呼吸道标本中的结核分枝杆菌复合群。
J Clin Microbiol. 2011 Apr;49(4):1621-3. doi: 10.1128/JCM.02214-10. Epub 2011 Feb 2.
6
Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system.应用自动化 GeneXpert MTB/RIF 系统快速检测肺外结核。
J Clin Microbiol. 2011 Apr;49(4):1202-5. doi: 10.1128/JCM.02268-10. Epub 2011 Jan 26.
7
Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.采用整合实时 PCR 方法快速检测涂阴临床标本中的结核分枝杆菌复合群和利福平耐药性。
J Clin Microbiol. 2011 Mar;49(3):1137-9. doi: 10.1128/JCM.01831-10. Epub 2010 Dec 29.
8
Xpert(®) MTB/RIF for point-of-care diagnosis of TB in high-HIV burden, resource-limited countries: hype or hope?Xpert(®) MTB/RIF 即时检测系统在高 HIV 负担、资源有限国家的结核病点检测诊断：炒作还是希望？
Expert Rev Mol Diagn. 2010 Oct;10(7):937-46. doi: 10.1586/erm.10.67.
9
Rapid molecular detection of tuberculosis and rifampin resistance.快速分子检测结核分枝杆菌及利福平耐药性。
N Engl J Med. 2010 Sep 9;363(11):1005-15. doi: 10.1056/NEJMoa0907847. Epub 2010 Sep 1.
10
Evaluation of the analytical performance of the Xpert MTB/RIF assay.评估 Xpert MTB/RIF assay 的分析性能。
J Clin Microbiol. 2010 Jul;48(7):2495-501. doi: 10.1128/JCM.00128-10. Epub 2010 May 26.

Xpert MTB/RIF 外部质量评估干培养物斑：南非第一阶段试点研究结果。

Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

机构信息

Department of Molecular Medicine and Haematology, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, South Africa.

出版信息

J Clin Microbiol. 2011 Dec;49(12):4356-60. doi: 10.1128/JCM.05167-11. Epub 2011 Oct 5.

DOI:10.1128/JCM.05167-11
PMID:21976767
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3232964/
Abstract

Implementation of Xpert MTB/RIF requires quality assessment. A pilot program using dried culture spots (DCSs) of inactivated Mycobacterium tuberculosis is described. Of 274 DCS results received, 2.19% generated errors; the remainder yielded 100% correct Mycobacterium tuberculosis detection. The probe A cycle threshold (C(T)) variability of three DCS batches was ≤ 3.47. The study of longer-term DCS stability is ongoing.

摘要

Xpert MTB/RIF 的实施需要质量评估。本文描述了一个使用已灭活结核分枝杆菌干燥培养物斑点（DCS）的试点项目。在收到的 274 份 DCS 结果中，有 2.19%出现错误；其余的结果均为 100%正确的结核分枝杆菌检测。三个 DCS 批次的探针 A 循环阈值（C(T)）变异性≤3.47。目前正在对 DCS 的长期稳定性进行研究。