Singh Sukhdeep, Acharya Anirudh B, Kumar S C Veerendra
Department of Periodontics, New Horizon Dental College and Research Institute, Sakri, Bilaspur, Chhattisgarh, India.
J Indian Soc Periodontol. 2011 Apr;15(2):152-5. doi: 10.4103/0972-124X.84385.
To evaluate neutrophil myeloperoxidase (MPO) staining procedure as a reliable, affordable and easily available diagnostic assay for aggressive periodontitis.
Fifteen subjects were recruited in the study wherein five each were diagnosed as aggressive periodontitis and chronic periodontitis respectively, and five were periodontally healthy. Three millilitres (ml) of venous blood was collected using Vacutainers containing ethylene diamine tetra acetate (EDTA) and was subjected to MPO staining procedure. Histological picture was evaluated using a visual analogue scale (VAS).
MPO stained specimen of all the patients showed positive MPO staining of the neutrophils. The intensity of the stain of MPO granules was more in aggressive periodontitis specimen as compared to the chronic periodontitis patient specimen and healthy subject specimen. The staining characteristics were comparable for chronic periodontitis patients and healthy subject.
This study shows that there is a potential and probable place for MPO staining as an economical, relatively convenient and easily available assay in the diagnosis of aggressive periodontitis.
评估中性粒细胞髓过氧化物酶（MPO）染色程序作为侵袭性牙周炎一种可靠、经济且易于获得的诊断检测方法的可行性。
本研究招募了15名受试者，其中5名分别被诊断为侵袭性牙周炎和慢性牙周炎，另外5名牙周健康。使用含有乙二胺四乙酸（EDTA）的真空采血管采集3毫升静脉血，并进行MPO染色程序。使用视觉模拟评分法（VAS）评估组织学图像。
所有患者的MPO染色标本均显示中性粒细胞MPO染色呈阳性。与慢性牙周炎患者标本和健康受试者标本相比，侵袭性牙周炎标本中MPO颗粒的染色强度更高。慢性牙周炎患者和健康受试者的染色特征具有可比性。
本研究表明，MPO染色作为一种经济、相对便捷且易于获得的检测方法，在侵袭性牙周炎的诊断中具有潜在且可能的应用价值。
