速效救心丸（见试验）对实验性动脉粥样硬化大鼠氧化应激和炎症反应的影响。

Effects of suxiao jiuxin pill (see test) on oxidative stress and inflammatory response in rats with experimental atherosclerosis.

机构信息

Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300054, China.

出版信息

J Tradit Chin Med. 2011 Jun;31(2):107-11. doi: 10.1016/s0254-6272(11)60022-8.

DOI:10.1016/s0254-6272(11)60022-8

PMID:21977809

Abstract

OBJECTIVE

To observe the preventive role of Suxiao Jiuxin Pill (see text) on atherosclerosis (AS) and to probe into the mechanism in the atherosclerosis rat model.

METHODS

The AS rat model was established by a high fat diet and a large dose of calcium (vitamin D3, 0.6 million U/kg, i.p, once). Sixty healthy male adult Sprague-Dawlay (SD) rats were randomly divided into 6 groups, a normal control group (N), a model group (M), a SX low dose group (SXL), a SX middle dose group (SXM), a SX high dose group (SXH), and an atorvastatin group (ATO) (n = 10 in each group). The rats in the treatment groups were given with the specific drugs from the first day by oral administration, and the normal control group and the model group were given with normal saline for 12 weeks. Afterwards, the content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and the content of oxidized low density lipoprotein (ox-LDL) in the serum were detected. In addition, the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) proteins were tested by Western-blot method.

RESULTS

The serum ox-LDL and MDA level significantly decreased, SOD activity increased in the SX middle, high dose groups and the atorvastatin group compared to the model group (all P < 0.05). While the expression of PPARgamma and NF-kappab proteins significantly decreased in the SX low, middle, high dose groups and the atorvastatin group compared to the model group (all P < 0.01), with the best effect in the SX high dose group .These results indicate that SX could elevate the activity of serum SOD, decrease serum level of MDA and ox-LDL, and reduce the expression of PPARgamma and NF-kappaB proteins.

CONCLUSION

SX plays an important role in anti-inflammation and inhibition of oxidative stress, which possibly are the mechanism of its preventing and treating atherosclerosis.

摘要

目的

观察速效救心丸对动脉粥样硬化（AS）的预防作用，并探讨其在动脉粥样硬化大鼠模型中的作用机制。

方法

采用高脂饮食和大剂量钙（维生素 D3，60 万 U/kg，腹腔注射，1 次）建立 AS 大鼠模型。将 60 只健康雄性成年 SD 大鼠随机分为 6 组，正常对照组（N）、模型组（M）、速效救心丸低剂量组（SXL）、速效救心丸中剂量组（SXM）、速效救心丸高剂量组（SXH）和阿托伐他汀组（ATO）（每组 10 只）。治疗组大鼠从第 1 天开始灌胃给予特定药物，正常对照组和模型组给予生理盐水，共 12 周。然后检测血清中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和氧化型低密度脂蛋白（ox-LDL）含量。此外，采用 Western blot 法检测过氧化物酶体增殖物激活受体γ（PPARγ）和核因子κB（NF-κB）蛋白的表达。

结果

与模型组相比，SX 中、高剂量组和阿托伐他汀组血清 ox-LDL 和 MDA 水平显著降低，SOD 活性升高（均 P<0.05）。而 SX 低、中、高剂量组和阿托伐他汀组 PPARγ和 NF-κB 蛋白表达均显著降低（均 P<0.01），其中 SX 高剂量组效果最佳。这些结果表明，SX 可提高血清 SOD 活性，降低血清 MDA 和 ox-LDL 水平，降低 PPARγ和 NF-κB 蛋白表达。