Department of Orthopaedic Surgery, Wake Forest School of Medicine, Medical Center Boulevard, Winston Salem, North Carolina 27157, USA.
Microcirculation. 2011 Nov;18(8):663-9. doi: 10.1111/j.1549-8719.2011.00135.x.
Keratin proteins have been utilized as biomaterials for decades, and are currently under investigation for a variety of tissue regeneration and trauma applications. It has been suggested that certain keratins may have the capacity to act as a colloid in fluid resuscitation applications, providing viscosity and oncotic properties that may be beneficial during acute ischemic events. Oxidized keratin derivatives, also known as keratoses, show good blood and cardiovascular compatibility and thus are the subject of this study.
The effects of keratose compounds will be assessed using a topload i.v. infusion model and observation of changes in the microvasculature of the cremaster muscle of rats.
Keratose resuscitation fluid (KRF) administration resulted in significant vasodilation in the cremaster muscle. This effect was blocked with pretreatment of l-NA to inhibit NO. Another keratin fraction, alpha-keratose, which is the primary viscosic compound, was not found to induce vasodilation.
The apparent mechanism of vasodilation was found to be NO-mediated and isolated to a particular purified fraction, the KAP.
角蛋白蛋白已被用作生物材料数十年，目前正在研究用于各种组织再生和创伤应用。有人提出，某些角蛋白可能具有在液体复苏应用中充当胶体的能力，提供粘度和胶体渗透压特性，这在急性缺血事件中可能是有益的。氧化角蛋白衍生物，也称为角化病，显示出良好的血液和心血管相容性，因此是本研究的主题。
将使用顶置式静脉输注模型评估角蛋白化合物的作用，并观察大鼠提睾肌中小血管的变化。
角蛋白复苏液（KRF）的给药导致提睾肌显著扩张。用 l-NA 预处理以抑制 NO 可阻断此作用。另一种角蛋白部分，即主要粘性化合物的α-角蛋白，未发现可引起血管舒张。
发现血管舒张的明显机制是 NO 介导的，并且仅分离出一种特定的纯化部分，即 KAP。
