Poranki D, Goodwin C, Van Dyke M
Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA.
Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Biomed Res Int. 2016;2016:1803912. doi: 10.1155/2016/1803912. Epub 2016 Nov 29.
Partial thickness burns can advance to full thickness after initial injury due to inadequate tissue perfusion and increased production of inflammatory cytokines, which has been referred to as burn wound progression. In previous work, we demonstrated that a keratin biomaterial hydrogel appeared to reduce burn wound progression. In the present study, we tested the hypothesis that a modified keratin hydrogel could reduce burn wound progression and speed healing. Standardized burn wounds were created in Yorkshire swine and treated within 30 minutes with keratin hydrogel (modified and unmodified), collagen hydrogel, or silver sulfadiazine (SSD). Digital images of each wound were taken for area measurements immediately prior to cleaning and dressing changes. Wound tissue was collected and assessed histologically at several time points. Wound area showed a significant difference between hydrogels and SSD groups, and rates of reepithelialization at early time points showed an increase when keratin treatment was used compared to both collagen and SSD. A linear regression model predicted a time to wound closure of approximately 25 days for keratin hydrogel while SSD treatment required 35 days. There appeared to be no measurable differences between the modified and unmodified formulations of keratin hydrogels.
由于组织灌注不足和炎症细胞因子产生增加，部分厚度烧伤在初始损伤后可进展为全层烧伤，这被称为烧伤创面进展。在先前的研究中，我们证明了一种角蛋白生物材料水凝胶似乎可以减少烧伤创面进展。在本研究中，我们测试了一种改良角蛋白水凝胶可以减少烧伤创面进展并加速愈合的假设。在约克郡猪身上创建标准化烧伤创面，并在30分钟内用角蛋白水凝胶（改良和未改良）、胶原蛋白水凝胶或磺胺嘧啶银（SSD）进行治疗。在每次清洁和换药前立即拍摄每个伤口的数字图像以进行面积测量。在几个时间点收集伤口组织并进行组织学评估。水凝胶组和SSD组之间的伤口面积存在显著差异，与胶原蛋白和SSD相比，使用角蛋白治疗时早期的再上皮化率有所增加。线性回归模型预测角蛋白水凝胶的伤口闭合时间约为25天，而SSD治疗需要35天。角蛋白水凝胶的改良配方和未改良配方之间似乎没有可测量的差异。
