Department of Clinical and Chemical Pathology, Kasr El Aini Hospital, Cairo University, El Saraya Street, Infront of El Manial Palace, Cairo 11451, Egypt.
Leuk Res. 2012 Feb;36(2):169-73. doi: 10.1016/j.leukres.2011.08.017. Epub 2011 Oct 5.
Neuropilins are expressed in tumors vasculature and cells. Their expression is thought to be correlated with tumor angiogenesis and progression. In this study, we analyzed NRP-1 expression level in 40 acute leukemia patients [20 acute myeloid leukemia (AML) and 20 acute lymphoblastic leukemia (ALL)] and 10 healthy controls using Real-Time Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RTQ-PCR) aiming to show Neuropilin-1 (NRP-1) gene expression pattern in acute leukemia patients and its role in disease severity and progression. NRP-1 was expressed in 80% and 95% of ALL and AML respectively with levels higher in patients than controls and in ALL than AML patients. NRP-1 levels were significantly correlated with blast percentage and complete remission. We conclude that NRP-1 is significantly associated with acute leukemia and that its level might serve as an indicator for disease severity and progression. NRP-1 signaling may represent a novel therapeutic approach for the treatment of acute leukemia subsets.
神经纤毛蛋白在肿瘤血管和细胞中表达。它们的表达被认为与肿瘤血管生成和进展相关。在这项研究中，我们使用实时定量逆转录聚合酶链反应（RTQ-PCR）分析了 40 例急性白血病患者[20 例急性髓系白血病（AML）和 20 例急性淋巴细胞白血病（ALL）]和 10 例健康对照者的 NRP-1 表达水平，旨在显示急性白血病患者中神经纤毛蛋白-1（NRP-1）基因的表达模式及其在疾病严重程度和进展中的作用。NRP-1 在 ALL 和 AML 中分别表达 80%和 95%，患者中的表达水平高于对照组，在 ALL 中高于 AML 患者。NRP-1 水平与原始细胞百分比和完全缓解显著相关。我们得出结论，NRP-1 与急性白血病显著相关，其水平可能作为疾病严重程度和进展的指标。NRP-1 信号可能代表治疗急性白血病亚群的一种新的治疗方法。
