Instituto de Biotecnología - UNAM, Avenida Universidad, Colonia Chamilpa, Cuernavaca, Morelos, Mexico.
Toxicon. 2011 Dec 1;58(8):644-63. doi: 10.1016/j.toxicon.2011.09.015. Epub 2011 Sep 28.
This communication reviews most of the important findings related to venom components isolated from scorpions and spiders, mainly by means of gene cloning and expression. Rather than revising results obtained by classical biochemical studies that report structure and function of venom components, here the emphasis is placed on cloning and identification of genes present in the venomous glands of these arachnids. Aspects related to cDNA library construction, specific or random ESTs cloning, transcriptome analysis, high-throughput screening, heterologous expression and folding are briefly discussed, showing some numbers of species and components already identified, but also shortly mentioning limitations and perspectives of research for the future in this field.
本通讯综述了与通过基因克隆和表达从蝎子和蜘蛛中分离出的毒液成分相关的大多数重要发现。这里的重点不是修订通过报告毒液成分结构和功能的经典生化研究获得的结果，而是侧重于克隆和鉴定这些蛛形纲动物毒腺中存在的基因。本文简要讨论了 cDNA 文库构建、特异性或随机 EST 克隆、转录组分析、高通量筛选、异源表达和折叠等方面，展示了已经鉴定出的一些物种和成分，但也简要提到了该领域未来研究的局限性和展望。
