Grupo de Toxinología y Alternativas Farmacéuticas y Alimentarias, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Medellin 1226, Colombia.
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, Cuernavaca 62250, Mexico.
Toxins (Basel). 2023 Jul 2;15(7):436. doi: 10.3390/toxins15070436.
Spider venoms are composed, among other substances, of peptide toxins whose selectivity for certain physiological targets has made them powerful tools for applications such as bioinsecticides, analgesics, antiarrhythmics, antibacterials, antifungals and antimalarials, among others. Bioinsecticides are an environmentally friendly alternative to conventional agrochemicals. In this paper, the primary structure of an insecticidal peptide was obtained from the venom gland transcriptome of the ctenid spider (Transcript ID PhdNtxNav24). The peptide contains 53 amino acids, including 10 Cys residues that form 5 disulfide bonds. Using the amino acid sequence of such peptide, a synthetic gene was constructed de novo by overlapping PCRs and cloned into an expression vector. A recombinant peptide, named delta-ctenitoxin (rCtx-4), was obtained. It was expressed, folded, purified and validated using mass spectrometry (7994.61 Da). The insecticidal activity of rCtx-4 was demonstrated through intrathoracic injection in crickets (LD 1.2 μg/g insect) and it was not toxic to mice. rCtx-4 is a potential bioinsecticide that could have a broad spectrum of applications in agriculture.
蜘蛛毒液除其他物质外，还含有肽类毒素，这些毒素对某些生理靶标的选择性使它们成为生物杀虫剂、镇痛药、抗心律失常药、抗菌药、抗真菌药和抗疟药等应用的有力工具。生物杀虫剂是传统农用化学品的一种环保替代品。在本文中，从鳞翅目蜘蛛的毒液腺转录组中获得了一种杀虫肽的一级结构（转录 ID PhdNtxNav24）。该肽包含 53 个氨基酸，包括 10 个 Cys 残基，形成 5 个二硫键。使用该肽的氨基酸序列，通过重叠 PCR 从头构建了一个合成基因，并克隆到表达载体中。获得了一种名为 delta-ctenitoxin (rCtx-4) 的重组肽。它通过质荷比（7994.61 Da）测定法进行了表达、折叠、纯化和验证。通过对蟋蟀进行胸内注射（LD 1.2 μg/g 昆虫）证明了 rCtx-4 的杀虫活性，且对小鼠没有毒性。rCtx-4 是一种有潜力的生物杀虫剂，在农业中有广泛的应用前景。
