Castilla J A, Sampalo A, Molina R, Samaniego F, Mozas J, Vergara F, Garrido F, Herruzo A J
Servicio de Análisis Clínicos, Ciudad Sanitaria, Virgen de las Nieves, Granada, Spain.
Am J Reprod Immunol. 1990 Mar-Apr;22(3-4):127-9. doi: 10.1111/j.1600-0897.1990.tb00655.x.
The study of lymphocyte subsets from human follicular fluid (FF) provides an opportunity to evaluate immunological features of the ovary. We investigated the mononuclear cell subsets in FF and peripheral blood obtained at the time of laparoscopy from ten in vitro fertilization (IVF) patients. Midcycle nonpregnant peripheral blood was used as the control. A marked increase in the proportion of monocytes (CD14+) was observed in FF. Although FF was enriched with CD8+ lymphocytes, a decrease in the proportion of CD4+ lymphocytes was observed. "Memory" T cells in FF, identified by the CD4+ CD45R- phenotype, predominated over "naive" T cells (CD4+ CD45R+) at a ratio of 2:1, which differs from the ratio yielded by control blood samples (1:1). The percentage of activated T cells (CD3+ HLA-DR+ cell) increased significantly in FF. When lymphocyte subsets were studied in the peripheral blood of IVF patients, changes similar to but less significant than those in FF were found. These data support the concept that lymphocytes play an important role in ovarian physiology.
对人卵泡液（FF）中淋巴细胞亚群的研究为评估卵巢的免疫学特征提供了契机。我们调查了10名体外受精（IVF）患者在腹腔镜检查时获取的卵泡液和外周血中的单核细胞亚群。将月经周期中期未孕的外周血用作对照。在卵泡液中观察到单核细胞（CD14 +）比例显著增加。尽管卵泡液富含CD8 +淋巴细胞，但观察到CD4 +淋巴细胞比例下降。以CD4 + CD45R -表型鉴定的卵泡液中的“记忆”T细胞以2：1的比例超过“初始”T细胞（CD4 + CD45R +），这与对照血样的比例（1：1）不同。卵泡液中活化T细胞（CD3 + HLA - DR +细胞）的百分比显著增加。当研究IVF患者外周血中的淋巴细胞亚群时，发现了与卵泡液中相似但不太显著的变化。这些数据支持淋巴细胞在卵巢生理学中起重要作用这一概念。
