Mahde Atheer, Shaker Mahmud, Al-Mashhadani Zohair
Department of Acceptable Analysis, Health and Medical Technical College, Baghdad, Iraq.
Oman Med J. 2009 Apr;24(2):108-18. doi: 10.5001/omj.2009.25.
Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease with obesity. Currently, no data exists on the relative correlation between omentin-1 with other adipokines or the expression and regulation of omentin-1 in the serum of women with PCOS. The objective of this study is to evaluate the role of omentin-1 levels or omentin-1 /adipokines ratio in the serum of women with PCOS compared with matched control subjects.
The study involved 60 patients with PCOS and 30 women without PCOS who were used as controls. To examine the relationship between fasting serum omentin-1 and serum interleukin-6 (IL-6), interleukin-8 (IL-8), resistin, ghrelin, leptin RBP-4 and tumor necrosis factor-a (TNF-a) levels in infertile PCOS and non-PCOS subjects. Also, insulin and other hormones were measured in both groups. All these factors were measured by enzyme-linked immunosorbent assays.
From the total of 60 cases, there was a significant increase (p<0.001) in PCOS patients when compared to the control group in fasting serum, serum interleukin-6 (IL-6), interleukin-8 (IL-8), resistin, leptin RBP-4, tumor necrosis factor-a (TNF-a) levels and insulin. A significant decrease in omentin-1 and ghrelin (p<0.001) was observed. The results also showed that 93.33% and 98.30% in PCOS patients had abnormal omentin1: Insulin ratio and omentin1: Resistin ratio respectively according to the cut off values ≤27.42 and ≤31.35. Moreover, 81.67% of PCOS patients had abnormal omentin1:IL-6 ratio according to the cut of value (≤66.09).
This is the first time the role of plasma omentin1 has been investigated with respect to its implication in PCOS. Usually, LH/FSH ratio and FAI (ratio of total testosterone to SHBG) are the important factors used for the diagnosis of PCOS in all previous literature, but none of them referred to the parameters discussed in this report. Also, the percentage of sensitivity and the difference between range of these parameters in PCOS patients and the controls may give a different perspective in trying to understand the etiology of PCOS. Therefore, these parameters may be used for future diagnosis of PCOS. This study also suggested that omentin/resistin ratio may play a crucial paracrine or endocrine role in modulating insulin sensitivity.
多囊卵巢综合征（PCOS）与胰岛素抵抗和肥胖相关。最近的研究表明，血浆网膜素-1水平随肥胖而降低。目前，关于网膜素-1与其他脂肪因子之间的相对相关性，或PCOS女性血清中网膜素-1的表达及调控，尚无相关数据。本研究的目的是评估与匹配的对照受试者相比，PCOS女性血清中网膜素-1水平或网膜素-1/脂肪因子比值的作用。
本研究纳入60例PCOS患者和30例无PCOS的女性作为对照。检测不孕的PCOS和非PCOS受试者空腹血清网膜素-1与血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、抵抗素、胃饥饿素、瘦素、视黄醇结合蛋白4（RBP-4）和肿瘤坏死因子-α（TNF-α）水平之间的关系。同时，测量两组的胰岛素及其他激素水平。所有这些因子均采用酶联免疫吸附测定法检测。
在全部60例患者中，与对照组相比，PCOS患者空腹血清、血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、抵抗素、瘦素、RBP-4、肿瘤坏死因子-α（TNF-α）水平及胰岛素水平显著升高（p<0.001）。观察到网膜素-1和胃饥饿素显著降低（p<0.001）。结果还显示，根据截断值≤27.42和≤31.35，PCOS患者中分别有93.33%和98.30%的网膜素1:胰岛素比值和网膜素1:抵抗素比值异常。此外，根据截断值（≤66.09），81.67%的PCOS患者网膜素1:IL-6比值异常。
这是首次研究血浆网膜素-1在PCOS中的作用。以往所有文献中，通常使用LH/FSH比值和游离雄激素指数（FAI，总睾酮与性激素结合球蛋白的比值）作为诊断PCOS的重要因素，但均未提及本报告中讨论的参数。此外，PCOS患者和对照组中这些参数的敏感性百分比及范围差异，可能为理解PCOS的病因提供不同视角。因此，这些参数可用于未来PCOS的诊断。本研究还表明，网膜素/抵抗素比值可能在调节胰岛素敏感性方面发挥关键的旁分泌或内分泌作用。
