El-Fekhfakh Effat Abdel-Monaem, Hassanain Nawal Abdel-Hafiz, El-Folly Runia Fouad, El-Hariri Hazem
Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Egypt.
J Egypt Soc Parasitol. 2011 Aug;41(2):497-512.
A total of 30 patients suffering from brucellosis were suspected based on history taking, clinical manifestations and positive serum tube agglutination test (at titer > or = 1/160). The followings were done for all cases; complete blood picture (differential leucocytic count) and liver function tests, serodiagnosis of Brucella (serum tube agglutination test (STAT) as well as Rose Bengal test (RBT) and PCR. The study aimed to analyze the diagnostic value of RBT as compared to STAT and PCR for human brucellosis, and to evaluate the sensitivity, specificity, accuracy, the cost and the time consuming of RBT as compared to STAT and PCR. There was a significant difference between diagnosis by RBT and both STAT > or = 1/640, & STAT > or = 1/1280. Also, there was a significant difference between PCR and both STAT > or = 1/640, and STAT > or = 1/1280. No significant difference was detected between RBT in diagnosing acute and chronic infection. STAT > or = 1/320 proved to be better than STAT at other titers and RBT in diagnosis of brucellosis. RBT proved to be suitable as screening test regarding time (faster) and cost. But, STAT > or = 1/320 from a practical and economic point of views proved to be the best one in diagnosing human brucellosis.
根据病史采集、临床表现及血清试管凝集试验阳性（滴度≥1/160），共怀疑30例布氏杆菌病患者。对所有病例进行了以下检查：全血细胞计数（白细胞分类计数）和肝功能检查、布氏杆菌血清学诊断（血清试管凝集试验（STAT）以及虎红试验（RBT）和聚合酶链反应（PCR）。本研究旨在分析RBT与STAT和PCR相比对人类布氏杆菌病的诊断价值，并评估RBT与STAT和PCR相比的敏感性、特异性、准确性、成本及耗时。RBT与STAT≥1/640及STAT≥1/1280诊断结果之间存在显著差异。此外，PCR与STAT≥1/640及STAT≥1/1280诊断结果之间也存在显著差异。RBT在诊断急性和慢性感染方面未检测到显著差异。在布氏杆菌病诊断中，STAT≥1/320在其他滴度时比STAT及RBT表现更好。RBT在时间（更快）和成本方面被证明适合作为筛查试验。但是，从实际和经济角度来看，STAT≥1/320在诊断人类布氏杆菌病方面被证明是最佳的。
