Siegfried L, Puzová H, Hlaucová J, Durovicová J, Krocková J, Ebert H H
Katedra hygieny, epidemiológie a mikrobiológie, Srobárová, Kosice.
Bratisl Lek Listy. 1990 Apr;91(4):292-7.
Killing of 61 seroresistant E. coli strains equipped with virulence plasmids (F, Ent, Hly, Col) and resistance plasmids (R) in polymorphonuclear leukocytes (PMNL) of healthy donors was determined by the cultivation plate and fluorescence methods of phagocytosis. Killing of the control E. coli strain K12 which does not possess any plasmids was studied by the fluorescent method. Compared to the fluorescent method, the cultivation plate method yielded a significantly higher killing rate of the bacteria after opsonization by 5% (p less than 0.05) and concentrated serum (p less than 0.001). Compared to the control E. coli strain K12, the killing rate of seroresistant E. coli strains in PMNL of healthy donors was significantly lower (p less than 0.001).
通过培养平板法和吞噬作用荧光法，测定健康供体多形核白细胞（PMNL）对61株携带毒力质粒（F、Ent、Hly、Col）和耐药质粒（R）的血清抗性大肠杆菌菌株的杀灭情况。采用荧光法研究了不携带任何质粒的对照大肠杆菌菌株K12的杀灭情况。与荧光法相比，培养平板法在经5%调理素（p<0.05）和浓缩血清（p<0.001）调理后，细菌的杀灭率显著更高。与对照大肠杆菌菌株K12相比，健康供体PMNL中血清抗性大肠杆菌菌株的杀灭率显著更低（p<0.001）。
