Hanner Robert, Floyd Robin, Bernard Andrea, Collette Bruce B, Shivji Mahmood
Biodiversity Institute of Ontario & Department of Integrative Biology, University of Guelph, 50 Stone Road East, Guelph, ON, Canada N1G 2W1.
Mitochondrial DNA. 2011 Oct;22 Suppl 1:27-36. doi: 10.3109/19401736.2011.596833.
DNA barcoding is a method promising fast and accurate identification of animal species based on the sequencing of the mitochondrial c oxidase subunit (COI) gene. In this study, we explore the prospects for DNA barcoding in one particular fish group, the billfishes (suborder Xiphioidei--swordfish, marlins, spearfishes, and sailfish). We sequenced the mitochondrial COI gene from 296 individuals from the 10 currently recognized species of billfishes, and combined these data with a further 57 sequences from previously published projects. We also sequenced the rhodopsin gene from a subset of 72 individuals to allow comparison of mitochondrial results against a nuclear marker. Five of the 10 species are readily distinguishable by COI barcodes. Of the rest, the striped marlin (Kajikia audax) and white marlin (K. albida) show highly similar sequences and are not unambiguously distinguishable by barcodes alone, likewise are the three spearfishes Tetrapturus angustirostris, T. belone, and T. pfluegeri. We discuss the taxonomic status of these species groups in light of our and other data, molecular and morphological.
DNA条形码是一种基于线粒体细胞色素c氧化酶亚基（COI）基因测序，有望快速准确鉴定动物物种的方法。在本研究中，我们探讨了DNA条形码在一个特定鱼类群体——旗鱼（剑鱼亚目，包括剑鱼、枪鱼、四鳍旗鱼和旗鱼）中的应用前景。我们对目前已知的10种旗鱼的296个个体的线粒体COI基因进行了测序，并将这些数据与之前发表项目中的另外57个序列相结合。我们还对72个个体的视紫红质基因进行了测序，以便将线粒体结果与核标记进行比较。10个物种中有5个很容易通过COI条形码区分开来。其余的物种中，条纹枪鱼（Kajikia audax）和白马林鱼（K. albida）的序列高度相似，仅靠条形码无法明确区分，同样，三种四鳍旗鱼（Tetrapturus angustirostris、T. belone和T. pfluegeri）也是如此。我们根据我们的数据以及其他分子和形态学数据，讨论了这些物种组的分类地位。
