Filonzi Laura, Ardenghi Alessia, Rontani Pietro Maria, Voccia Andrea, Ferrari Claudio, Papa Riccardo, Bellin Nicolò, Nonnis Marzano Francesco
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 43124 Parma, Italy.
Department Biology, University of Puerto Rico, Rio Piedras, San Juan 00925, Puerto Rico.
Foods. 2023 Jun 20;12(12):2420. doi: 10.3390/foods12122420.
The recent increase in international fish trade leads to the need for improving the traceability of fishery products. In relation to this, consistent monitoring of the production chain focusing on technological developments, handling, processing and distribution via global networks is necessary. Molecular barcoding has therefore been suggested as the gold standard in seafood species traceability and labelling. This review describes the DNA barcoding methodology for preventing food fraud and adulteration in fish. In particular, attention has been focused on the application of molecular techniques to determine the identity and authenticity of fish products, to discriminate the presence of different species in processed seafood and to characterize raw materials undergoing food industry processes. In this regard, we herein present a large number of studies performed in different countries, showing the most reliable DNA barcodes for species identification based on both mitochondrial (, , rDNA and rDNA) and nuclear genes. Results are discussed considering the advantages and disadvantages of the different techniques in relation to different scientific issues. Special regard has been dedicated to a dual approach referring to both the consumer's health and the conservation of threatened species, with a special focus on the feasibility of the different genetic and genomic approaches in relation to both scientific objectives and permissible costs to obtain reliable traceability.
近期国际鱼类贸易的增长导致了提高渔业产品可追溯性的需求。与此相关的是,有必要通过全球网络对生产链进行持续监测,重点关注技术发展、处理、加工和分销。因此,分子条形码技术已被建议作为海产品物种可追溯性和标签的黄金标准。本综述描述了用于防止鱼类食品欺诈和掺假的DNA条形码方法。特别关注了分子技术在确定鱼类产品的身份和真实性、辨别加工海产品中不同物种的存在以及表征食品工业加工过程中的原材料方面的应用。在这方面,我们在此展示了在不同国家进行的大量研究,这些研究展示了基于线粒体(,,rDNA和rDNA)和核基因的用于物种鉴定的最可靠DNA条形码。考虑到不同技术在不同科学问题上的优缺点,对结果进行了讨论。特别关注了涉及消费者健康和濒危物种保护的双重方法,特别关注不同遗传和基因组方法在实现科学目标和获得可靠可追溯性的允许成本方面的可行性。