Key Laboratory of Forest Plant Ecology, Northeast Forestry Universit, Ministry of Education, Harbin 150040, PR China.
Nat Prod Res. 2012;26(9):842-9. doi: 10.1080/14786419.2011.558016. Epub 2011 Oct 7.
This study was designed to examine the composition and in vitro antioxidant activity of the essential oil of Schisandra chinensis fruits. Gas chromatography/mass spectrometry was used to determine the composition of the essential oil. Forty components were identified, representing 90.80% of the oil; ylangene (37.72%), β-himachalene (10.46%) and α-bergamotene (8.57%) were the main components. Antioxidant and radical-scavenging properties were evaluated by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and β-carotene bleaching assay. In the DPPH assay, IC(50) value of the S. chinensis essential oil was determined as 4.17 mg mL(-1). In the β-carotene bleaching assay, the essential oil (1.0 mg mL(-1)) exhibited 25.89% inhibition against linoleic acid oxidation. In both systems, antioxidant capacity of butylated hydroxytoluene was also determined in parallel experiments.
本研究旨在考察五味子果实精油的组成和体外抗氧化活性。采用气相色谱/质谱联用技术测定了精油的组成。鉴定出 40 种成分,占油的 90.80%;榄香醇(37.72%)、β-扁柏烯(10.46%)和α-佛手柑烯(8.57%)为主要成分。通过 2,2-二苯基-1-苦基肼(DPPH)法和β-胡萝卜素漂白法评估了抗氧化和自由基清除性能。在 DPPH 法中,五味子精油的 IC50 值为 4.17 mg mL(-1)。在β-胡萝卜素漂白法中,精油(1.0 mg mL(-1))对亚油酸氧化的抑制率为 25.89%。在这两种体系中,也在平行实验中测定了丁基羟基甲苯的抗氧化能力。