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五味子果实精油的化学成分和抗氧化活性。

Chemical composition and antioxidant activity of the essential oil of Schisandra chinensis fruits.

机构信息

Key Laboratory of Forest Plant Ecology, Northeast Forestry Universit, Ministry of Education, Harbin 150040, PR China.

出版信息

Nat Prod Res. 2012;26(9):842-9. doi: 10.1080/14786419.2011.558016. Epub 2011 Oct 7.

Abstract

This study was designed to examine the composition and in vitro antioxidant activity of the essential oil of Schisandra chinensis fruits. Gas chromatography/mass spectrometry was used to determine the composition of the essential oil. Forty components were identified, representing 90.80% of the oil; ylangene (37.72%), β-himachalene (10.46%) and α-bergamotene (8.57%) were the main components. Antioxidant and radical-scavenging properties were evaluated by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and β-carotene bleaching assay. In the DPPH assay, IC(50) value of the S. chinensis essential oil was determined as 4.17 mg  mL(-1). In the β-carotene bleaching assay, the essential oil (1.0 mg mL(-1)) exhibited 25.89% inhibition against linoleic acid oxidation. In both systems, antioxidant capacity of butylated hydroxytoluene was also determined in parallel experiments.

摘要

本研究旨在考察五味子果实精油的组成和体外抗氧化活性。采用气相色谱/质谱联用技术测定了精油的组成。鉴定出 40 种成分,占油的 90.80%;榄香醇(37.72%)、β-扁柏烯(10.46%)和α-佛手柑烯(8.57%)为主要成分。通过 2,2-二苯基-1-苦基肼(DPPH)法和β-胡萝卜素漂白法评估了抗氧化和自由基清除性能。在 DPPH 法中,五味子精油的 IC50 值为 4.17 mg mL(-1)。在β-胡萝卜素漂白法中,精油(1.0 mg mL(-1))对亚油酸氧化的抑制率为 25.89%。在这两种体系中,也在平行实验中测定了丁基羟基甲苯的抗氧化能力。

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