Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Tokyo 113-8656, Japan.
Colloids Surf B Biointerfaces. 2012 Nov 1;99:1-6. doi: 10.1016/j.colsurfb.2011.08.029. Epub 2011 Sep 8.
Non-invasive and effective cell recovery from culture substrates is important for the passage and characterization of cells. In this study, a photoreactive polymer surface, which uses UV-irradiation to control substrate cell adhesion, was prepared. The photoreactive phospholipid polymer (PMB-PL) reported herein, was composed of a both 2-methacryloyloxyethyl phosphorylcholine (MPC) unit as a cytocompatible unit and methacrylate bearing a photolabile nitrobenzyl group. The PMB-PL polymer was used to coat a cell culture substrate thus affording a photoreactive surface. Surface analysis of the PMB-PL coating indicated a strong photoresponse owing to the sensitivity of the PL unit. Before light exposure, the PMB-PL surface provided cell adhesion. Following UV-irradiation, the PMB-PL coating was converted to a neutral ζ-potential and hydrophilic surface. The photoreactive surface conversion process allowed for the detachment of adhered cells from the PMB-PL surface while maintaining cell viability. This study demonstrates the promise and significance of the PMB-PL photoreactive surface as a method to control cell attachment and detachment for cell function investigation.
从培养基板上非侵入性且有效地回收细胞对于细胞的传代和特性鉴定非常重要。在本研究中，我们制备了一种光反应性聚合物表面，该表面利用紫外线照射来控制基板上细胞的黏附。本文报道的光反应性磷脂聚合物（PMB-PL）由既具有亲水性又具有细胞相容性的 2-甲基丙烯酰氧乙基磷酸胆碱（MPC）单元和带有光不稳定的硝基苄基的甲基丙烯酰基组成。PMB-PL 聚合物被用于涂覆细胞培养基板，从而提供了一种光反应性表面。PMB-PL 涂层的表面分析表明，由于 PL 单元的敏感性，其具有很强的光响应性。在光暴露之前，PMB-PL 表面提供了细胞黏附。经紫外线照射后，PMB-PL 涂层转换为中性 ζ 电位和亲水表面。光反应性表面的转换过程允许黏附的细胞从 PMB-PL 表面脱离，同时保持细胞活力。本研究证明了 PMB-PL 光反应性表面作为一种控制细胞黏附和脱离以研究细胞功能的方法具有潜力和重要性。
