全面分析干细胞相关因子在肾细胞癌中的临床意义。
Comprehensive analysis of clinical significance of stem-cell related factors in renal cell cancer.
机构信息
Department of Urology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
出版信息
World J Surg Oncol. 2011 Oct 7;9:121. doi: 10.1186/1477-7819-9-121.
BACKGROUND
C-MYC, LIN28, OCT4, KLF4, NANOG and SOX2 are stem cell related factors. We detected whether these factors express in renal cell carcinoma (RCC) tissues to study their correlations with the clinical and pathological characteristics.
METHODS
The expressions of c-MYC, LIN28, SOX2, KLF4, OCT4 and NANOG in 30 RCC patients and 5 non-RCC patients were detected with quantitative real-time reverse transcription-PCR (qRT-PCR). The data were analyzed with Wilcoxon signed rank sum test and x2 test.
RESULTS
In RCC group, c-MYC expression was significantly higher in RCC tissues compared with normal tissues (P < 0.05). The expression levels of OCT4, KLF4, NANOG and SOX2 were significantly lower in RCC tissues compared with normal tissues (P < 0.05). LIN28 expression level was not significant. No difference was observed when it comes to clinical and pathological characteristics such as gender, age, tumor size, cTNM classification and differentiation status (P > 0.05). Also the expression levels of all above factors were not significantly changed in non-RCC group (P > 0.05).
CONCLUSIONS
The present analysis strongly suggests that altered expression of several stem cell related factors may play different roles in RCC. C-MYC may function as an oncogene and OCT4, KLF4, NANOG and SOX2 as tumor suppressors.
背景
C-MYC、LIN28、OCT4、KLF4、NANOG 和 SOX2 是与干细胞相关的因子。我们检测了这些因子在肾细胞癌（RCC）组织中的表达情况，以研究它们与临床和病理特征的相关性。
方法
采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 30 例 RCC 患者和 5 例非 RCC 患者的 C-MYC、LIN28、SOX2、KLF4、OCT4 和 NANOG 表达。采用 Wilcoxon 符号秩和检验和 x2 检验对数据进行分析。
结果
在 RCC 组中，与正常组织相比，RCC 组织中 C-MYC 的表达明显升高（P<0.05）。OCT4、KLF4、NANOG 和 SOX2 的表达水平在 RCC 组织中明显低于正常组织（P<0.05）。LIN28 的表达水平无差异。性别、年龄、肿瘤大小、cTNM 分期和分化程度等临床病理特征无差异（P>0.05）。同样，非 RCC 组各因素的表达水平也无明显变化（P>0.05）。
结论
本分析强烈表明，几个与干细胞相关的因子的表达改变可能在 RCC 中发挥不同的作用。C-MYC 可能作为癌基因，OCT4、KLF4、NANOG 和 SOX2 可能作为肿瘤抑制因子。
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