Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
J Fluoresc. 2010 Jan;20(1):275-81. doi: 10.1007/s10895-009-0551-2. Epub 2009 Oct 13.
Simple and sensitive spectrophotometric and spectrofluorimetric methods have been developed for the determination of hydrochlorothiazide (I), indapamide (II) and xipamide(III) based on ternary complex formation with eosin and lead (II) in the presence of methylcellulose as surfactant. The methods do not involve solvent extraction. For spectrophotometric method, the ternary complex showed an absorption maximum at 543 nm. The factors affecting the formation of ternary complex were studied and optimized. The method obeys Beer's law over concentration range of 8-40 microg mL(-1). A fluorescence quenching method for the determination of the cited drugs by forming this ternary complex was also investigated for the purpose of enhancing the sensitivity of the determination. The analytical performance of both methods was fully validated, and the results were satisfactory. The methods have been successfully applied for the determination of the studied drugs in their pharmaceutical tablets and the results obtained ware in good agreement with those obtained by the reference method. Common excipients used as additives in tablets do not interfere with the proposed methods.
已经开发出了简单灵敏的分光光度法和荧光光度法，用于基于曙红和铅（II）与甲基纤维素作为表面活性剂形成的三元配合物，来测定氢氯噻嗪（I）、吲达帕胺（II）和西帕胺（III）。该方法不涉及溶剂萃取。对于分光光度法，三元配合物在 543nm 处显示出最大吸收。研究并优化了影响三元配合物形成的因素。该方法在 8-40μg mL(-1)浓度范围内符合比尔定律。还研究了通过形成这种三元配合物来测定这些药物的荧光猝灭法，以提高测定的灵敏度。充分验证了两种方法的分析性能，结果令人满意。这些方法已成功应用于测定其药物片剂中的研究药物，所得结果与参考方法所得结果吻合良好。片剂中作为添加剂使用的常见赋形剂不干扰所提出的方法。
