Departamento de Protección Vegetal, Instituto de Ciencias Agrarias (ICA, CSIC), C/Serrano 115 dpdo, 28006 Madrid, Spain.
Arthropod Struct Dev. 2012 Jan;41(1):79-86. doi: 10.1016/j.asd.2011.07.005. Epub 2011 Oct 6.
Scanning (SEM) and transmission (TEM) electron microscopy were used to elucidate the morphology of the rostrum, as well as the mandibular and maxillary stylets of the psyllid Diaphorina citri, vector of phloem-inhabiting bacteria associated with citrus huanglongbing (HLB) disease. D. citri has a cone-shaped rostrum that extends behind the pair of prothoracic coxae. The stylet bundle comprises a pair of mandibular (Md) and maxillary (Mx) stylets with a mean length of 513.3 μm; when retracted, their proximal portions form a loop and are stored in the crumena (Cr). Serial cross-sections of the rostrum revealed that the mandibles are always projected in front of the maxillary stylets. The two maxillary stylets form the food and salivary canals, with diameters of 0.9 μm and 0.4 μm respectively. These two canals merge at the end of the stylets forming a common duct with a length of 4.3 μm and a mean diameter of 0.9 μm. The acrostyle, a distinct anatomical structure present in the common duct of aphid maxillary stylets, was not observed by TEM in the ultrathin cross-sections of the common duct (CD) of D. citri. This study provides new information on D. citri mouthparts that may help to understand the feeding behaviour of this important vector of HLB-associated bacteria.
扫描电子显微镜（SEM）和透射电子显微镜（TEM）用于阐明橘蚜的喙、下颚和上颚喙的形态，橘蚜是与柑橘黄龙病相关的韧皮部细菌的载体。橘蚜的喙呈锥形，延伸到前两对胸足之后。喙束由一对下颚（Md）和上颚（Mx）喙组成，平均长度为 513.3μm；当缩回时，它们的近端部分形成一个环，并储存在 crumena（Cr）中。喙的连续横切片显示，下颚总是在前部突出于上颚喙之外。两个上颚喙形成食物和唾液道，直径分别为 0.9μm 和 0.4μm。这两个通道在喙的末端合并，形成一个长 4.3μm、平均直径为 0.9μm 的共同管道。在 TEM 观察橘蚜的普通管超薄片时，未观察到上颚喙普通管中存在的明显解剖结构——acrostyl。本研究提供了有关橘蚜口器的新信息，这可能有助于理解该重要HLB 相关细菌载体的取食行为。
