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利用微计算机断层扫描技术揭示成年柑橘木虱（昆虫纲：半翅目，木虱科）的解剖结构及其在柑橘叶片内刺穿和取食的方式。

Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf.

机构信息

Department of Zoology, Faculty of Sciences, University of Granada, Campus de Fuentenueva, Granada, Spain.

U.S. Dept. Agriculture, Agricultural Research Service, Fort Pierce, FL, USA.

出版信息

Sci Rep. 2021 Jan 14;11(1):1358. doi: 10.1038/s41598-020-80404-z.

DOI:10.1038/s41598-020-80404-z
PMID:33446699
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7809155/
Abstract

The Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

摘要

柑橘木虱（ACP），Diaphorina citri，是柑橘树的有害害虫，它传播 Candidatus Liberibacter spp.，导致黄龙病（HLB）（柑橘绿病）；这被认为是柑橘植物最严重的细菌性疾病。在这里，我们详细描述了使用微计算机断层扫描（micro-CT）的外部和内部解剖结构（不包括生殖系统）的解剖学研究。这是对木虱昆虫解剖结构的第一次完整的 3D 微 CT 重建，包括对成年木虱在柑橘叶上取食的 3D 重建，可在移动设备上使用。详细的渲染图像和视频支持对 coxal 和 scapus 触角腺以及内部解剖结构（后肠、中胸神经节和大脑）的性差异的首次描述。这代表了我们对 ACP 解剖结构的认识，以及对木虱的认识的重大进展。这些图像、视频和 3D 模型共同构成了一个独特的解剖图谱，是未来研究和教学辅助的有用工具。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e577/7809155/21b556ed5fd2/41598_2020_80404_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e577/7809155/b15472923b36/41598_2020_80404_Fig11_HTML.jpg
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6
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