Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands.
Leuk Res. 2012 Apr;36(4):422-30. doi: 10.1016/j.leukres.2011.09.015. Epub 2011 Oct 6.
Flow cytometry (FC) is recognized as an important tool in the diagnosis of myelodysplastic syndromes (MDS) especially when standard criteria fail. A working group within the Dutch Society of Cytometry aimed to implement FC in the diagnostic work-up of MDS. Hereto, guidelines for data acquisition, analysis and interpretation were formulated. Based on discussions on analyses of list mode data files and fresh MDS bone marrow samples and recent literature, the guidelines were modified. Over the years (2005-2011), the concordance between the participating centers increased indicating that the proposed guidelines contributed to a more objective, standardized FC analysis, thereby ratifying the implementation of FC in MDS.
流式细胞术（FC）被认为是诊断骨髓增生异常综合征（MDS）的重要工具，尤其是在标准标准失败时。荷兰细胞术协会内的一个工作组旨在将 FC 应用于 MDS 的诊断工作中。为此，制定了数据采集、分析和解释的指南。基于对列表模式数据文件和新鲜 MDS 骨髓样本的分析以及最新文献的讨论，对指南进行了修改。多年来（2005-2011 年），参与中心之间的一致性增加，表明所提出的指南有助于更客观、标准化的 FC 分析，从而批准将 FC 应用于 MDS。
