Department of Chemistry, University of Tennessee, Knoxville, TN, USA.
J Chromatogr A. 2011 Nov 11;1218(45):8255-63. doi: 10.1016/j.chroma.2011.08.088. Epub 2011 Sep 22.
Several simple techniques are presented for the identification of the boundaries of chromatographic peaks. These methods provide a significant reduction in the time needed to perform the rapid, automatic calculation of the central peak moments and to evaluate the quality of a separation while improving the accuracy of the measurements of column efficiencies. It was found that the identification of the peak boundaries as functions of the peak widths and the examination of the slope of the signal to noise versus time plot are viable alternatives to a manual determination.
介绍了几种简单的技术，用于确定色谱峰的边界。这些方法显著减少了快速自动计算中心峰矩和评估分离质量所需的时间，同时提高了柱效测量的准确性。结果发现，将峰边界作为峰宽的函数进行识别，并检查信号与噪声随时间的斜率，是手动确定的可行替代方法。
