Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Food Chem Toxicol. 2011 Dec;49(12):3272-8. doi: 10.1016/j.fct.2011.09.022. Epub 2011 Sep 29.
Black soybean (Glycine max) has been used for traditional medicine and food in Asian countries, but safety of its hull has not been studied. We conducted acute and chronic oral toxicity studies. For the acute study, an extract of black soybean hull (BE; 2.5 g/kg body weight) was administered singly by intragastric intubation to Sprague-Dawley rats and C57BL/6 mice. There was no death or significant decrease in body weight in rats and mice, and the oral LD(50) of BE was >2.5 g/kg body weight. In the chronic study, BE was administered at dietary levels of 0% (control), 2.0%, and 5.0% to male and female C57BL/6 mice for 26 weeks. No mortality or toxicologically significant clinical changes were observed through the experimental period. Although body weights, as well as abdominal fat, blood levels of triglyceride and total cholesterol in 5.0% males were significantly lower than that in control and 2.0% groups, these changes were considered not to be adverse. Hematology and histopathological observation revealed no toxicologically significant changes. The no-observed adverse-effect-level of BE was estimated to be 5.0% in the diet (5074.1 mg/kg body weight/day for males and 7617.9 mg/kg body weight/day for females).
黑豆（Glycine max）在亚洲国家被用于传统医学和食品，但尚未对其豆壳的安全性进行研究。我们进行了急性和慢性口服毒性研究。对于急性研究，通过胃内插管单独给予黑豆壳提取物（BE；2.5 g/kg 体重）给 Sprague-Dawley 大鼠和 C57BL/6 小鼠。大鼠和小鼠均无死亡或体重显著下降，BE 的口服 LD50 ＞2.5 g/kg 体重。在慢性研究中，将 BE 以 0%（对照）、2.0%和 5.0%的饮食水平给予雄性和雌性 C57BL/6 小鼠 26 周。在整个实验期间，未观察到死亡率或毒理学上有意义的临床变化。尽管 5.0%雄性的体重以及腹部脂肪、甘油三酯和总胆固醇的血液水平明显低于对照和 2.0%组，但这些变化被认为没有不良影响。血液学和组织病理学观察未显示出毒理学上有意义的变化。BE 的无观察到不良效应水平估计为饮食中的 5.0%（雄性 5074.1 mg/kg 体重/天，雌性 7617.9 mg/kg 体重/天）。
