Yam Mun Fei, Sadikun Amirin, Ahmad Mariam, Akowuah Gabriel Akyirem, Asmawi Mohd Zaini
Falkultas Farmasi, Universitas Sumatera Utara, 5 Jalan Almamater, USU-Kampus, Medan 20155, Indonesia.
J Ethnopharmacol. 2009 Jun 22;123(2):244-9. doi: 10.1016/j.jep.2009.03.011. Epub 2009 Mar 24.
Gynura procumbens (Merr.), which is known as "Sambung nyawa", is widely used in South East Asian countries in the traditional treatment of many ailments. However, there is little toxicological information available regarding safety following repeated exposure. The present investigation describes the toxicity of a methanol extract of Gynura procumbens leaves in rats.
For acute toxicity studies, a methanol extract of Gynura procumbens was orally administered to Sprague-Dawley rats (female and male) at a dose range of 1000-5000 mg/kg. For sub-chronic toxicity studies, the rats were orally administered the methanol extract of Gynura procumbens at the doses of 125, 250, and 500 mg/(kg day) for a period of 13 weeks. The animals were sacrificed, followed by examination of their organs and blood serum.
Administration of the methanol extract from Gynura procumbens leaves at 1000-5000 mg/kg did not produce mortality or significant changes in the general behaviour, body weight, or organ gross appearance of rats. There were no significant differences in the general condition, growth, organ weights, haematological parameters, clinical chemistry values, or gross and microscopic appearance of the organs from the treatment groups as compared to the control group. Therefore, the NOAEL for the Gynura procumbens methanol extract is 500 mg/(kg day) administered orally for 13 weeks.
平卧菊三七，即“续命草”，在东南亚国家被广泛用于多种疾病的传统治疗。然而，关于反复接触后的安全性，毒理学信息却很少。本研究描述了平卧菊三七叶甲醇提取物对大鼠的毒性。
急性毒性研究中，将平卧菊三七甲醇提取物以1000 - 5000毫克/千克的剂量范围口服给予斯普拉格-道利大鼠（雌雄皆有）。亚慢性毒性研究中，大鼠以125、250和500毫克/（千克·天）的剂量口服给予平卧菊三七甲醇提取物，持续13周。处死动物后，对其器官和血清进行检查。
以1000 - 5000毫克/千克的剂量给予大鼠平卧菊三七叶甲醇提取物，未导致大鼠死亡，也未使其一般行为、体重或器官外观出现显著变化。与对照组相比，治疗组大鼠的一般状况、生长、器官重量、血液学参数、临床化学值以及器官的大体和微观外观均无显著差异。因此，平卧菊三七甲醇提取物经口给药13周的无观察到有害作用水平为500毫克/（千克·天）。
