Zheng N, Pan C, Liu W
Department of Gynaecology and Obstetrics, Tongde Hospital of Zhejiang Province, Hangzhou, China.
J Int Med Res. 2011;39(4):1184-92. doi: 10.1177/147323001103900406.
This study used proteomic fingerprint technology, combining nano-sized magnetic beads with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), to screen for potential protein biomarkers for the diagnosis of endometriosis. Serum proteins from 126 patients with endometriosis and 120 healthy controls were profiled and compared. Biomarker pattern software identified 46 discriminating mass-to-charge m/z ratio peaks that were related to endometriosis. The model constructed by the software, based on three of these peaks (m/z 5988.7, 7185.3 and 8929.8), generated excellent separation between the endometriosis and control groups. The sensitivity was 91.4% and the specificity 95.0%. Blind testing on a second series of serum samples from patients with endometriosis and healthy controls indicated a sensitivity of 89.3% and a specificity of 90.0%. Biomarkers for endometriosis can be discovered in serum by MALDI-TOF-MS in combination with nano-sized magnetic beads. The pattern of combined markers provides a powerful and reliable diagnostic method for endometriosis, with high sensitivity and specificity.
本研究采用蛋白质组指纹技术，将纳米磁珠与基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）相结合，以筛选用于诊断子宫内膜异位症的潜在蛋白质生物标志物。对126例子宫内膜异位症患者和120例健康对照者的血清蛋白进行了分析和比较。生物标志物模式软件识别出46个与子宫内膜异位症相关的具有鉴别意义的质荷比（m/z）峰。该软件基于其中三个峰（m/z 5988.7、7185.3和8929.8）构建的模型，在子宫内膜异位症组和对照组之间产生了良好的区分效果。灵敏度为91.4%，特异性为95.0%。对另一组来自子宫内膜异位症患者和健康对照者的血清样本进行盲法检测，结果显示灵敏度为89.3%，特异性为90.0%。通过MALDI-TOF-MS结合纳米磁珠可在血清中发现子宫内膜异位症的生物标志物。联合标志物模式为子宫内膜异位症提供了一种强大而可靠的诊断方法，具有高灵敏度和特异性。
