Yang L, Fang J S, Wang W, Chen R K, Shen C F
Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan, China.
J Int Med Res. 2011;39(4):1344-57. doi: 10.1177/147323001103900421.
This study investigated whether transplantation of Schwann cells differentiated from adipose-derived stem cells (ADSC-SCs) of rats could promote functional improvement after contusion brain injury, with a focus on the effect on reactive gliosis. ADSCs were isolated and expanded from groin adipose tissue of Sprague-Dawley rats and then differentiated into Schwann cells. ADSCSCs were transplanted into the contused rat brain. Immunofluorescence and Western blotting were used to analyse reactive gliosis, and locomotor function of the rats was assessed. Hemiparalysed rats transplanted with ADSC-SCs showed significant locomotor function recovery compared with rats transplanted with undifferentiated ADSCs or control rats injected with medium alone. Transplanted ADSC-SCs significantly reduced glial scar formation and neurocan protein levels compared with transplanted undifferentiated ADSCs. In conclusion, transplantation of ADSC-SCs can effectively promote locomotor functional recovery and reduce reactive gliosis after contusion brain injury in rats.
本研究调查了大鼠脂肪来源干细胞分化的雪旺细胞（ADSC-SCs）移植是否能促进脑挫伤后功能改善，重点关注对反应性胶质增生的影响。从Sprague-Dawley大鼠腹股沟脂肪组织中分离并扩增脂肪来源干细胞，然后将其分化为雪旺细胞。将ADSC-SCs移植到脑挫伤大鼠脑中。采用免疫荧光和蛋白质印迹法分析反应性胶质增生，并评估大鼠的运动功能。与移植未分化脂肪来源干细胞的大鼠或仅注射培养基的对照大鼠相比，移植ADSC-SCs的偏瘫大鼠运动功能有显著恢复。与移植未分化脂肪来源干细胞相比，移植ADSC-SCs显著减少了胶质瘢痕形成和神经黏蛋白水平。总之，ADSC-SCs移植可有效促进大鼠脑挫伤后运动功能恢复并减少反应性胶质增生。
