Research & Development Centre, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, Andhra Pradesh, India.
Vaccine. 2011 Nov 15;29(49):9201-8. doi: 10.1016/j.vaccine.2011.09.117. Epub 2011 Oct 8.
Coccidiosis is an economically important disease affecting poultry industry and remains one of the major problems globally. Developing a cost effective sub-unit vaccine may help mitigate loss in the industry. Here, we report expressing one of the microneme proteins, EtMIC2 from Eimeria tenella in tobacco using Agrobacterium-mediated transient expression. The ability of plant expressed recombinant EtMIC2 in eliciting both humoral and cell-mediated immune responses were measured in the immunized birds. The protective efficacy in the vaccinated birds against a homologous challenge was also evaluated. Birds immunized with plant expressed EtMIC2 showed good sero-conversion, reduced oocyst output and increased weight gain when compared to control birds. Our data indicate that use of plant expressed recombinant EtMIC2 in birds was safe and had the potential in imparting partial protection in chickens against homologous challenge.
球虫病是一种严重影响家禽养殖业的重要疾病，也是全球范围内的主要问题之一。开发一种具有成本效益的亚单位疫苗可能有助于减轻该行业的损失。在这里，我们报告了使用农杆菌介导的瞬时表达在烟草中表达艾美耳球虫的微线体蛋白 EtMIC2 的一种方法。在免疫鸟类中测量了植物表达的重组 EtMIC2 引发体液和细胞介导免疫反应的能力。还评估了接种疫苗的鸟类对同源性挑战的保护效力。与对照鸟类相比，用植物表达的 EtMIC2 免疫的鸟类显示出良好的血清转化率、减少卵囊产量和增加体重增加。我们的数据表明，在鸟类中使用植物表达的重组 EtMIC2 是安全的，并有可能在赋予鸡对同源性挑战的部分保护方面具有潜力。
