Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province 271018, China.
Vaccine. 2014 Apr 1;32(16):1869-76. doi: 10.1016/j.vaccine.2014.01.068. Epub 2014 Feb 11.
S. cerevisiae is generally regarded as safe and benign organism and its surface display system may be used as a unique eukaryotic expression system that is suitable for expressing eukaryotic antigen. In addition to the convenience of vaccine delivery, the yeast cell wall has been shown to enhance the innate immunity when immunized with the yeast live oral vaccine. In the present study, we expressed the chicken coccidian E. tenella EtMic2, a microneme protein, on the surface of the S. cerevisiae and evaluated it as a potential oral vaccine for chicken against E. tenella challenge. The protective efficacy against a homologous challenge was evaluated by body weight gains, lesion scores and fecal oocyst shedding. The results showed that the live oral vaccine can improve weight gains, reduced cecal pathology and lower oocyst fecal shedding compared with non immunized controls. In addition, the yeast oral vaccine could stimulate humoral as well as cell mediate immune responses. These results suggested that EtMic2 displayed on the cell surface of S. cerevisiae could be used as potential live vaccine against chicken coccidiosis.
酿酒酵母通常被认为是安全和良性的生物体，其表面展示系统可用作适合表达真核抗原的独特真核表达系统。除了疫苗传递的便利性之外，酵母细胞壁已被证明在用酵母活口服疫苗免疫时可增强先天免疫。在本研究中，我们在酿酒酵母表面表达了鸡球虫 E. tenella 的 EtMic2，一种微线蛋白，评估其作为针对 E. tenella 攻毒的鸡潜在口服疫苗。通过体重增加、病变评分和粪便卵囊脱落来评估针对同源攻毒的保护效力。结果表明，与未免疫对照相比，活口服疫苗可提高体重增加、降低盲肠病理学和降低粪便卵囊脱落。此外，酵母口服疫苗可刺激体液和细胞介导的免疫反应。这些结果表明，在酿酒酵母细胞表面展示的 EtMic2 可作为针对鸡球虫病的潜在活疫苗。
