Research & Development Centre, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, Andhra Pradesh, India.
Vaccine. 2012 Jun 22;30(30):4460-4. doi: 10.1016/j.vaccine.2012.04.076. Epub 2012 May 1.
Coccidiosis is a disease caused by intracellular parasites belonging to the genus Eimeria. In the present study, we transiently expressed two coccidial antigens EtMIC1 and EtMIC2 as poly histidine-tagged fusion proteins in tobacco. We have evaluated the protective efficacy of plant expressed EtMIC1 as monovalent and as well as bi-valent formulation where EtMIC1 and EtMIC2 were used in combination. The protective efficacy of these formulations was evaluated using homologous challenge in chickens. We observed better serum antibody response, weight gain and reduced oocyst shedding in birds immunized with EtMIC1 and EtMIC2 as bivalent formulation compared to monovalent formulation. However, IFN-γ response was not significant in birds immunized with EtMIC1 compared to the birds immunized with EtMIC2. Our results indicate the potential use of these antigens as vaccine candidates.
球虫病是一种由属于艾美耳属的细胞内寄生虫引起的疾病。在本研究中，我们将两种球虫抗原 EtMIC1 和 EtMIC2 作为聚组氨酸标记的融合蛋白在烟草中瞬时表达。我们评估了植物表达的 EtMIC1 的单价和双价制剂的保护效力，其中 EtMIC1 和 EtMIC2 联合使用。使用同源性挑战在鸡中评估这些制剂的保护效力。与单价制剂相比，用 EtMIC1 和 EtMIC2 双价制剂免疫的鸡观察到更好的血清抗体反应、体重增加和卵囊脱落减少。然而，与用 EtMIC2 免疫的鸡相比，用 EtMIC1 免疫的鸡 IFN-γ 反应不显著。我们的结果表明这些抗原有作为疫苗候选物的潜力。
