利用 PEG/盐基双水相体系直接从芒果（Mangifera Indica Cv. Chokanan）皮中提取果胶酶。

Direct purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel using a PEG/salt-based Aqueous Two Phase System.

机构信息

Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

出版信息

Molecules. 2011 Oct 10;16(10):8419-27. doi: 10.3390/molecules16108419.

DOI:10.3390/molecules16108419

PMID:21986520

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6264526/

Abstract

An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral salts (0-8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.

摘要

首次采用双水相体系（ATPS）从芒果皮（Mangifera indica Cv. Chokanan）中分离和纯化果胶酶。考察了不同参数对果胶酶分配行为的影响，如聚合物分子量（聚乙二醇，2000-10000）、磷酸钾组成（12-20%，w/w）、体系 pH（6-9）和添加不同浓度中性盐（0-8%，w/w）。酶的分配系数随 PEG 分子量的增加而降低。此外，相组成对酶的纯化因子和产率有显著影响。在 pH 7.0 下，在 14% PEG 4000-14%磷酸钾系统中添加 3%（w/w）NaCl 可实现从芒果皮中纯化果胶酶的最佳条件。在此体系下，果胶酶的纯化因子提高到 13.2，产率高达 97.6%。因此，本研究证明 ATPS 可以作为一种从芒果皮中分配果胶酶的廉价有效方法。