Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Clin Biochem. 2011 Dec;44(17-18):1385-9. doi: 10.1016/j.clinbiochem.2011.09.014. Epub 2011 Oct 2.
To investigate the relationship between carbonic anhydrase (CA) II autoantibody and lipid peroxidation, certain antioxidant parameters, and cytokines in rheumatoid arthritis (RA) patients.
Serum levels of CA II autoantibody, cytokines (TNFα, IL-6, IFN-γ, IL-1β) and bone markers (crosslaps, osteocalcine) and erythrocyte levels of antioxidant enzyme activities (SOD, CAT, GPx), GSH and MDA, and CA activities were measured in RA patients and healthy controls.
The CA II autoantibody titers were significantly higher (P<0.05), and erythrocyte SOD activities were significantly lower (P<0.05) in RA patients. A significant negative correlation between CA II autoantibody titers and SOD activities in RA group was established (r=-0.430, p=0.006). The elevated cytokine levels could not be correlated with CA II autoantibody levels in RA.
These results suggest that increased erythrocyte oxidative stress observed in RA may be effective in the mechanism of CA II autoantibody formation.
研究类风湿关节炎（RA）患者碳酸酐酶（CA）II 自身抗体与脂质过氧化、某些抗氧化参数和细胞因子之间的关系。
测量 RA 患者和健康对照组的血清 CA II 自身抗体、细胞因子（TNFα、IL-6、IFN-γ、IL-1β）和骨标志物（交联环、骨钙素）以及红细胞抗氧化酶活性（SOD、CAT、GPx）、GSH 和 MDA 和 CA 活性。
RA 患者的 CA II 自身抗体滴度明显升高（P<0.05），红细胞 SOD 活性明显降低（P<0.05）。在 RA 组中建立了 CA II 自身抗体滴度与 SOD 活性之间的显著负相关（r=-0.430，p=0.006）。升高的细胞因子水平与 RA 中的 CA II 自身抗体水平无法相关。
这些结果表明，在 RA 中观察到的红细胞氧化应激增加可能在 CA II 自身抗体形成的机制中起作用。
