INM - Leibniz Institute for New Materials, Nano Cell Interactions Group, Saarbrücken, Germany.
J Biophotonics. 2012 Feb;5(2):117-27. doi: 10.1002/jbio.201100064. Epub 2011 Oct 11.
The interactions of nanoparticles with human cells are of large interest in the context of nanomaterial safety. Here, we use live cell imaging and image-based fluorescence correlation methods to determine colocalization of 88 nm and 32 nm silica nanoparticles with endocytotic vesicles derived from the cytoplasmic membrane and lysosomes, as well as to quantify intracellular mobility of internalized particles, in contrast to particle number quantification by counting techniques. In our study, A549 cells are used as a model for human type II alveolar epithelial cells. We present data supporting endocytotic uptake of the particles and subsequent active transport to the perinuclear region. The presence of particles in lamellar bodies is proposed as a potential exocytosis route.
纳米颗粒与人类细胞的相互作用是纳米材料安全性研究的重要内容。在这里，我们使用活细胞成像和基于图像的荧光相关方法来确定 88nm 和 32nm 二氧化硅纳米颗粒与细胞质膜和溶酶体衍生的内吞小泡的共定位，以及定量内化颗粒的细胞内迁移率，与计数技术的颗粒数量定量形成对比。在我们的研究中，A549 细胞被用作人类 II 型肺泡上皮细胞的模型。我们提供的数据支持颗粒的内吞作用以及随后向核周区的主动运输。提出颗粒在板层小体中的存在是一种潜在的胞吐途径。
