Erasmus Medical Center, Department of Otorhinolaryngology Head and Neck Surgery, Rotterdam, The Netherlands.
Head Neck. 2012 Jul;34(7):1002-8. doi: 10.1002/hed.21861. Epub 2011 Oct 10.
In oral cancer surgery, intraoperative optical imaging could help the surgeon to determine adequate tumor-free margins.
Tumor-specific near-infrared fluorescence agents targeting epidermal growth factor receptor (CW800 EGF) or glucose transporter system (CW800 2-DG) were administered to mice with tongue carcinoma and cervical lymph node metastases. Tumor growth was followed by bioluminescence imaging. Fluorescence signals were compared with a control group of healthy animals.
Significantly higher fluorescence was found in tongue tumors and cervical lymph node metastases compared with that in control animals. Fluorescence correlated with histopathology. Tumor-to-background ratio of CW800 EGF in the tongue was 13.8 (SD = 6.1) and in the lymph nodes 15.7 (SD = 8.8). For CW800 2-DG, the tumor-to-background ratio in the tongue was 4.6 (SD = 2.1) and in the lymph nodes 33.9 (SD = 18.4).
Optical imaging can be used to detect oral cancer and cervical lymph node metastases and could potentially improve complete surgical resection by real-time image-guided surgery.
口腔癌手术中，术中光学成像是帮助外科医生确定无肿瘤安全切缘的有效方法。
给患有舌癌和颈部淋巴结转移的小鼠施用针对表皮生长因子受体（CW800 EGF）或葡萄糖转运系统（CW800 2-DG）的肿瘤特异性近红外荧光剂。通过生物发光成像来监测肿瘤的生长情况。将荧光信号与健康动物的对照组进行比较。
与对照组动物相比，舌肿瘤和颈部淋巴结转移处的荧光强度明显更高。荧光信号与组织病理学相关。CW800 EGF 在舌部的肿瘤与背景的比值为 13.8（SD=6.1），在淋巴结中的比值为 15.7（SD=8.8）。对于 CW800 2-DG，在舌部的肿瘤与背景的比值为 4.6（SD=2.1），在淋巴结中的比值为 33.9（SD=18.4）。
光学成像是一种可以用于检测口腔癌和颈部淋巴结转移的方法，它可能通过实时图像引导手术来提高完全切除率。
