Department of Preventive Medicine, Infectious Disease Clinical Research Program, Uniformed Services University, Bethesda, MD 20814, USA.
J Infect Dis. 2011 Nov;204 Suppl 3:S1000-10. doi: 10.1093/infdis/jir337.
Nine rhesus macaques were implanted with multisensor telemetry devices and internal jugular vein catheters before being infected with Zaire ebolavirus. All animals developed viremia, fever, a hemorrhagic rash, and typical changes of Ebola hemorrhagic fever in clinical laboratory tests. Three macaques unexpectedly survived this usually lethal disease, making it possible to compare physiological parameters in lethally challenged animals and survivors. After the onset of fever, lethal illness was characterized by a decline in mean arterial blood pressure, an increase in pulse and respiratory rate, lactic acidosis, and renal failure. Survivors showed less pronounced change in these parameters. Four macaques were randomized to receive supplemental volumes of intravenous normal saline when they became hypotensive. Although those animals had less severe renal compromise, no apparent survival benefit was observed. This is the first report of continuous physiologic monitoring in filovirus-infected nonhuman primates and the first to attempt cardiovascular support with intravenous fluids.
九只恒河猴在感染扎伊尔埃博拉病毒之前被植入了多传感器遥测设备和颈内静脉导管。所有动物均出现病毒血症、发热、出血性皮疹和埃博拉出血热的临床实验室检测典型变化。三只猴子出人意料地从这种通常致命的疾病中存活下来，这使得比较致死性挑战动物和幸存者的生理参数成为可能。发热后，致死性疾病的特征是平均动脉血压下降、脉搏和呼吸频率增加、酸中毒和肾功能衰竭。幸存者的这些参数变化不那么明显。四只猴子在低血压时被随机分配接受静脉注射生理盐水的补充量。尽管这些动物的肾功能损害较轻，但没有观察到明显的生存获益。这是首次在感染丝状病毒的非人类灵长类动物中进行连续生理监测的报告，也是首次尝试用静脉补液进行心血管支持。
