Ahmed Hayam S, Moawad Abeer S, AbouZid Sameh F, Owis Asmaa I
Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62111, Egypt.
Saudi Pharm J. 2020 May;28(5):593-598. doi: 10.1016/j.jsps.2020.03.011. Epub 2020 Apr 2.
(L.) Gaertn. (Asteraceae) was hydroponically cultured using a nutrient film technique system. Silibinin, isosilibinin and silychristin were detected in the fruits of the cultured plants. The effect of salicylic acid on the improvement of flavonolignans production by the fruits of the hydroponically cultured . was investigated. Salicylic acid was added to the nutrient solution at different concentrations (100, 200 and 400 µM) and the mature fruits of the plant were collected five days after elicitor addition. The fruits were then analyzed for their total flavonolignans contents and individual components using quantitative proton nuclear magnetic resonance spectroscopy (qHNMR) and high-performance liquid chromatography (HPLC). The results showed that elicitation with salicylic acid at 200 µM for five days increased production of total flavonolignans (1.7-fold by qHNMR and 1.6-fold by HPLC) higher than the control cultures and (1.4-fold by qHNMR and 1.1-fold by HPLC) higher than the cultivated plants. Silychristin was the major flavonolignan produced by the cultured plant. Elicitation by 200 µM salicylic acid increased silychristin production (1.6-fold by qHNMR and HPLC) higher than the control cultures and (1.3-fold by qHNMR and 1.0-fold by HPLC) higher than the cultivated plants. The present study provides a chance to improve secondary metabolite yield, serves as a useful tool for studying the biosynthesis of these medicinally valuable compounds and its regulation in plant and spots more light on hydroponic system as an important agricultural technique.
(L.) Gaertn.（菊科）采用营养液膜技术系统进行水培。在培养植物的果实中检测到水飞蓟宾、异水飞蓟宾和水飞蓟素。研究了水杨酸对水培植物果实中黄酮木脂素产量提高的影响。将不同浓度（100、200和400 μM）的水杨酸添加到营养液中，并在添加诱导剂五天后收集植物的成熟果实。然后使用定量质子核磁共振光谱（qHNMR）和高效液相色谱（HPLC）分析果实中的总黄酮木脂素含量和单个成分。结果表明，用200 μM水杨酸诱导五天，总黄酮木脂素的产量（通过qHNMR提高1.7倍，通过HPLC提高1.6倍）高于对照培养物，（通过qHNMR提高1.4倍，通过HPLC提高1.1倍）高于栽培植物。水飞蓟素是培养植物产生的主要黄酮木脂素。200 μM水杨酸诱导使水飞蓟素产量增加（通过qHNMR和HPLC提高1.6倍）高于对照培养物，（通过qHNMR提高1.3倍，通过HPLC提高1.0倍）高于栽培植物。本研究为提高次生代谢产物产量提供了机会，是研究这些具有药用价值化合物的生物合成及其在植物中的调控的有用工具，并为作为一种重要农业技术的水培系统提供了更多的启示。
