Santana M, Batista M, Alamo D, Cabrera F, González F, Gracia A
Unit of Reproduction and Obstetrics, Veterinary Faculty, University of Las Palmas de Gran Canaria, Arucas, Las Palmas, Spain.
Reprod Domest Anim. 2012 Jun;47(3):e43-6. doi: 10.1111/j.1439-0531.2011.01923.x. Epub 2011 Oct 12.
The influence of sexual stimulation and human chorionic gonadotrophin (hCG) administration on plasma testosterone concentrations was assessed in five male Beagles. Each dog was exposed to three experimental treatments: C treatment (Control, no stimulation), hCG treatment (dogs were SC injected with 1000 IU of hCG) and sexually stimulated (SS) treatment where semen was collected from the males. All dogs were exposed to all treatments, one per week for three consecutive weeks, with a 1 week of rest between treatments. Blood samples were taken with the same time intervals (0, 10, 30, 60 and 120 min) relative to treatments. Plasma testosterone concentrations were determined with a solid-phase I(125) radioimmunoassay. In the control treatment, the testosterone plasma levels did not show significant changes throughout the tested period (mean values ranging between 2.8 and 4.7 ng/ml); the hCG group presented a significant increase (p < 0.05) in plasma testosterone levels 30 min after hCG administration and had the highest value (8.7 ng/ml) at 120 min post-hCG. Finally, the SS group revealed a slight reduction in testosterone concentration immediately after ejaculation, but the values remained nearly unaltered until 120 min after semen collection. When the groups were compared, the hCG group showed higher plasma testosterone values (p < 0.05) than did the C and SS groups, starting at 30 min and continuing until the end of sampling. This study demonstrates that sexual stimulation associated with semen collection does not produce transitory modifications in plasma testosterone concentrations.
在五只雄性比格犬中评估了性刺激和注射人绒毛膜促性腺激素（hCG）对血浆睾酮浓度的影响。每只犬都接受三种实验处理：C处理（对照，无刺激）、hCG处理（犬皮下注射1000 IU的hCG）和性刺激（SS）处理，即从雄性犬采集精液。所有犬都接受所有处理，连续三周每周进行一种处理，处理之间休息1周。在相对于处理的相同时间间隔（0、10、30、60和120分钟）采集血样。采用固相I(125)放射免疫分析法测定血浆睾酮浓度。在对照处理中，整个测试期间睾酮血浆水平未显示出显著变化（平均值在2.8至4.7 ng/ml之间）；hCG组在注射hCG后30分钟血浆睾酮水平显著升高（p<0.05），并在注射hCG后120分钟达到最高值（8.7 ng/ml）。最后，SS组在射精后立即显示睾酮浓度略有降低，但在采集精液后120分钟内该值几乎保持不变。当对各组进行比较时，hCG组从30分钟开始直至采样结束，其血浆睾酮值均高于C组和SS组（p<0.05）。本研究表明，与精液采集相关的性刺激不会使血浆睾酮浓度产生短暂变化。
